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A protocol to measure slow protein dynamics of the cholera toxin B pentamers using broadband dielectric spectroscopy
The present protocol describes how to measure experimentally the slow protein dynamics that take place upon the thermal unfolding of the B subunit cholera toxin pentamers using broadband dielectric spectroscopy (BDS) in weakly hydrated and nanoconfined conditions. Transient unfolding intermediates,...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304676/ https://www.ncbi.nlm.nih.gov/pubmed/35874473 http://dx.doi.org/10.1016/j.xpro.2022.101561 |