Genomic characterization of high‐risk Escherichia coli and Enterobacter hormaechei clones recovered from a single tertiary‐care hospital in Pakistan

AIMS: Spread of carbapenem‐resistant Enterobacterales have become a global problem. We characterized extended‐spectrum β‐lactamase (ESBL)‐producing Enterobacterales from urinary tract infections cases from Allied Hospital Faisalabad, Pakistan. METHODS AND RESULTS: Eleven (22%, 11/50) ESBL‐producing...

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Autores principales: Mohsin, Mashkoor, Hassan, Brekhna, Khan, Ali Usman, Ali, Arslan, Swedberg, Göte, Hasan, Badrul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306472/
https://www.ncbi.nlm.nih.gov/pubmed/35137479
http://dx.doi.org/10.1111/jam.15482
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author Mohsin, Mashkoor
Hassan, Brekhna
Khan, Ali Usman
Ali, Arslan
Swedberg, Göte
Hasan, Badrul
author_facet Mohsin, Mashkoor
Hassan, Brekhna
Khan, Ali Usman
Ali, Arslan
Swedberg, Göte
Hasan, Badrul
author_sort Mohsin, Mashkoor
collection PubMed
description AIMS: Spread of carbapenem‐resistant Enterobacterales have become a global problem. We characterized extended‐spectrum β‐lactamase (ESBL)‐producing Enterobacterales from urinary tract infections cases from Allied Hospital Faisalabad, Pakistan. METHODS AND RESULTS: Eleven (22%, 11/50) ESBL‐producing Enterobacterales (Escherichia coli; n = 10 and Enterobacter hormaechei; n = 1) were recovered and processed through VITEK‐2, PCR, rep‐PCR followed by whole‐genome sequencing (WGS) of ESBL‐producing Ent. hormaechei and carbapenem‐resistant E. coli isolates. Plasmid transferability of bla (NDM‐1)‐producers was assayed by conjugation experiments. All ESBL strains carried the bla (CTX‐M‐15) gene. Of these bla (CTX‐M‐15) producing E. coli, four also carried bla (NDM‐1) located on transferable plasmids. All E. coli strains belonged to ST448 and displayed similar genetic features including genes for antimicrobial resistance, heavy metal, biocides and virulence. Genomic features of a multidrug‐resistant (MDR) Ent. hormaechei were also reported for the first time in Pakistan. CONCLUSION: Our findings indicate that bla (NDM‐1) producing E. coli ST448 is a multidrug, heavy metals and biocides‐resistant strain. Therefore, the screening of these isolates may be effective in limiting the MDR bacteria spread in hospitalized patients and within the community. SIGNIFICANCE AND IMPACT OF THIS STUDY: Spread of multi‐drug‐resistant ESBL‐producing bacteria in the clinical settings of Pakistan is a serious challenge and further limiting treatment options in the country. WGS could be used as a tool in the nationwide antibiotic surveillance programme to explore insights of spread and outbreak.
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spelling pubmed-93064722022-07-28 Genomic characterization of high‐risk Escherichia coli and Enterobacter hormaechei clones recovered from a single tertiary‐care hospital in Pakistan Mohsin, Mashkoor Hassan, Brekhna Khan, Ali Usman Ali, Arslan Swedberg, Göte Hasan, Badrul J Appl Microbiol Original Articles AIMS: Spread of carbapenem‐resistant Enterobacterales have become a global problem. We characterized extended‐spectrum β‐lactamase (ESBL)‐producing Enterobacterales from urinary tract infections cases from Allied Hospital Faisalabad, Pakistan. METHODS AND RESULTS: Eleven (22%, 11/50) ESBL‐producing Enterobacterales (Escherichia coli; n = 10 and Enterobacter hormaechei; n = 1) were recovered and processed through VITEK‐2, PCR, rep‐PCR followed by whole‐genome sequencing (WGS) of ESBL‐producing Ent. hormaechei and carbapenem‐resistant E. coli isolates. Plasmid transferability of bla (NDM‐1)‐producers was assayed by conjugation experiments. All ESBL strains carried the bla (CTX‐M‐15) gene. Of these bla (CTX‐M‐15) producing E. coli, four also carried bla (NDM‐1) located on transferable plasmids. All E. coli strains belonged to ST448 and displayed similar genetic features including genes for antimicrobial resistance, heavy metal, biocides and virulence. Genomic features of a multidrug‐resistant (MDR) Ent. hormaechei were also reported for the first time in Pakistan. CONCLUSION: Our findings indicate that bla (NDM‐1) producing E. coli ST448 is a multidrug, heavy metals and biocides‐resistant strain. Therefore, the screening of these isolates may be effective in limiting the MDR bacteria spread in hospitalized patients and within the community. SIGNIFICANCE AND IMPACT OF THIS STUDY: Spread of multi‐drug‐resistant ESBL‐producing bacteria in the clinical settings of Pakistan is a serious challenge and further limiting treatment options in the country. WGS could be used as a tool in the nationwide antibiotic surveillance programme to explore insights of spread and outbreak. John Wiley and Sons Inc. 2022-02-21 2022-05 /pmc/articles/PMC9306472/ /pubmed/35137479 http://dx.doi.org/10.1111/jam.15482 Text en © 2022 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Mohsin, Mashkoor
Hassan, Brekhna
Khan, Ali Usman
Ali, Arslan
Swedberg, Göte
Hasan, Badrul
Genomic characterization of high‐risk Escherichia coli and Enterobacter hormaechei clones recovered from a single tertiary‐care hospital in Pakistan
title Genomic characterization of high‐risk Escherichia coli and Enterobacter hormaechei clones recovered from a single tertiary‐care hospital in Pakistan
title_full Genomic characterization of high‐risk Escherichia coli and Enterobacter hormaechei clones recovered from a single tertiary‐care hospital in Pakistan
title_fullStr Genomic characterization of high‐risk Escherichia coli and Enterobacter hormaechei clones recovered from a single tertiary‐care hospital in Pakistan
title_full_unstemmed Genomic characterization of high‐risk Escherichia coli and Enterobacter hormaechei clones recovered from a single tertiary‐care hospital in Pakistan
title_short Genomic characterization of high‐risk Escherichia coli and Enterobacter hormaechei clones recovered from a single tertiary‐care hospital in Pakistan
title_sort genomic characterization of high‐risk escherichia coli and enterobacter hormaechei clones recovered from a single tertiary‐care hospital in pakistan
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306472/
https://www.ncbi.nlm.nih.gov/pubmed/35137479
http://dx.doi.org/10.1111/jam.15482
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