Optimized Hemolysin Type 1 Secretion System in Escherichia coli by Directed Evolution of the Hly Enhancer Fragment and Including a Terminator Region

Type 1 secretion systems (T1SS) have a relatively simple architecture compared to other classes of secretion systems and therefore, are attractive to be optimized by protein engineering. Here, we report a KnowVolution campaign for the hemolysin (Hly) enhancer fragment, an untranslated region upstrea...

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Detalles Bibliográficos
Autores principales: Pourhassan N., Zohreh, Cui, Haiyang, Khosa, Sakshi, Davari, Mehdi D., Jaeger, Karl‐Erich, Smits, Sander H. J., Schwaneberg, Ulrich, Schmitt, Lutz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306574/
https://www.ncbi.nlm.nih.gov/pubmed/35062047
http://dx.doi.org/10.1002/cbic.202100702

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306574/
https://www.ncbi.nlm.nih.gov/pubmed/35062047
http://dx.doi.org/10.1002/cbic.202100702

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