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Optimized Hemolysin Type 1 Secretion System in Escherichia coli by Directed Evolution of the Hly Enhancer Fragment and Including a Terminator Region
Type 1 secretion systems (T1SS) have a relatively simple architecture compared to other classes of secretion systems and therefore, are attractive to be optimized by protein engineering. Here, we report a KnowVolution campaign for the hemolysin (Hly) enhancer fragment, an untranslated region upstrea...
Autores principales: | Pourhassan N., Zohreh, Cui, Haiyang, Khosa, Sakshi, Davari, Mehdi D., Jaeger, Karl‐Erich, Smits, Sander H. J., Schwaneberg, Ulrich, Schmitt, Lutz |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306574/ https://www.ncbi.nlm.nih.gov/pubmed/35062047 http://dx.doi.org/10.1002/cbic.202100702 |
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