Gene Fusion and Directed Evolution to Break Structural Symmetry and Boost Catalysis by an Oligomeric C−C Bond‐Forming Enzyme

Gene duplication and fusion are among the primary natural processes that generate new proteins from simpler ancestors. Here we adopted this strategy to evolve a promiscuous homohexameric 4‐oxalocrotonate tautomerase (4‐OT) into an efficient biocatalyst for enantioselective Michael reactions. We firs...

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Detalles Bibliográficos
Autores principales: Xu, Guangcai, Kunzendorf, Andreas, Crotti, Michele, Rozeboom, Henriëtte J., Thunnissen, Andy‐Mark W. H., Poelarends, Gerrit J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306753/
https://www.ncbi.nlm.nih.gov/pubmed/34890491
http://dx.doi.org/10.1002/anie.202113970

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306753/
https://www.ncbi.nlm.nih.gov/pubmed/34890491
http://dx.doi.org/10.1002/anie.202113970

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