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Gene Fusion and Directed Evolution to Break Structural Symmetry and Boost Catalysis by an Oligomeric C−C Bond‐Forming Enzyme
Gene duplication and fusion are among the primary natural processes that generate new proteins from simpler ancestors. Here we adopted this strategy to evolve a promiscuous homohexameric 4‐oxalocrotonate tautomerase (4‐OT) into an efficient biocatalyst for enantioselective Michael reactions. We firs...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306753/ https://www.ncbi.nlm.nih.gov/pubmed/34890491 http://dx.doi.org/10.1002/anie.202113970 |