Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells

RPE tissues are derived from induced pluripotent stem cells (iPSCs) to model retinal diseases and as a replacement therapy for macular degeneration. Here, we developed a robust and efficient directed differentiation protocol to generate pure RPE cells that form a polarized monolayer. This protocol d...

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Detalles Bibliográficos
Autores principales: Sharma, Ruchi, Bose, Devika, Montford, Jair, Ortolan, Davide, Bharti, Kapil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307589/
https://www.ncbi.nlm.nih.gov/pubmed/35880133
http://dx.doi.org/10.1016/j.xpro.2022.101582
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author Sharma, Ruchi
Bose, Devika
Montford, Jair
Ortolan, Davide
Bharti, Kapil
author_facet Sharma, Ruchi
Bose, Devika
Montford, Jair
Ortolan, Davide
Bharti, Kapil
author_sort Sharma, Ruchi
collection PubMed
description RPE tissues are derived from induced pluripotent stem cells (iPSCs) to model retinal diseases and as a replacement therapy for macular degeneration. Here, we developed a robust and efficient directed differentiation protocol to generate pure RPE cells that form a polarized monolayer. This protocol describes how to set up RPE differentiation and to obtain a pure population that expresses mature RPE markers and forms strong tight junctions. For complete details on the use and execution of this protocol, please refer to Sharma et al., 2019, Sharma et al., 2021 and Miyagishima et al. (2021).
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spelling pubmed-93075892022-07-24 Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells Sharma, Ruchi Bose, Devika Montford, Jair Ortolan, Davide Bharti, Kapil STAR Protoc Protocol RPE tissues are derived from induced pluripotent stem cells (iPSCs) to model retinal diseases and as a replacement therapy for macular degeneration. Here, we developed a robust and efficient directed differentiation protocol to generate pure RPE cells that form a polarized monolayer. This protocol describes how to set up RPE differentiation and to obtain a pure population that expresses mature RPE markers and forms strong tight junctions. For complete details on the use and execution of this protocol, please refer to Sharma et al., 2019, Sharma et al., 2021 and Miyagishima et al. (2021). Elsevier 2022-07-20 /pmc/articles/PMC9307589/ /pubmed/35880133 http://dx.doi.org/10.1016/j.xpro.2022.101582 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sharma, Ruchi
Bose, Devika
Montford, Jair
Ortolan, Davide
Bharti, Kapil
Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells
title Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells
title_full Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells
title_fullStr Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells
title_full_unstemmed Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells
title_short Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells
title_sort triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307589/
https://www.ncbi.nlm.nih.gov/pubmed/35880133
http://dx.doi.org/10.1016/j.xpro.2022.101582
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