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Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells
RPE tissues are derived from induced pluripotent stem cells (iPSCs) to model retinal diseases and as a replacement therapy for macular degeneration. Here, we developed a robust and efficient directed differentiation protocol to generate pure RPE cells that form a polarized monolayer. This protocol d...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307589/ https://www.ncbi.nlm.nih.gov/pubmed/35880133 http://dx.doi.org/10.1016/j.xpro.2022.101582 |
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author | Sharma, Ruchi Bose, Devika Montford, Jair Ortolan, Davide Bharti, Kapil |
author_facet | Sharma, Ruchi Bose, Devika Montford, Jair Ortolan, Davide Bharti, Kapil |
author_sort | Sharma, Ruchi |
collection | PubMed |
description | RPE tissues are derived from induced pluripotent stem cells (iPSCs) to model retinal diseases and as a replacement therapy for macular degeneration. Here, we developed a robust and efficient directed differentiation protocol to generate pure RPE cells that form a polarized monolayer. This protocol describes how to set up RPE differentiation and to obtain a pure population that expresses mature RPE markers and forms strong tight junctions. For complete details on the use and execution of this protocol, please refer to Sharma et al., 2019, Sharma et al., 2021 and Miyagishima et al. (2021). |
format | Online Article Text |
id | pubmed-9307589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-93075892022-07-24 Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells Sharma, Ruchi Bose, Devika Montford, Jair Ortolan, Davide Bharti, Kapil STAR Protoc Protocol RPE tissues are derived from induced pluripotent stem cells (iPSCs) to model retinal diseases and as a replacement therapy for macular degeneration. Here, we developed a robust and efficient directed differentiation protocol to generate pure RPE cells that form a polarized monolayer. This protocol describes how to set up RPE differentiation and to obtain a pure population that expresses mature RPE markers and forms strong tight junctions. For complete details on the use and execution of this protocol, please refer to Sharma et al., 2019, Sharma et al., 2021 and Miyagishima et al. (2021). Elsevier 2022-07-20 /pmc/articles/PMC9307589/ /pubmed/35880133 http://dx.doi.org/10.1016/j.xpro.2022.101582 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Sharma, Ruchi Bose, Devika Montford, Jair Ortolan, Davide Bharti, Kapil Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells |
title | Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells |
title_full | Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells |
title_fullStr | Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells |
title_full_unstemmed | Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells |
title_short | Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells |
title_sort | triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307589/ https://www.ncbi.nlm.nih.gov/pubmed/35880133 http://dx.doi.org/10.1016/j.xpro.2022.101582 |
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