Dissociation and inDrops microfluidic encapsulation of human gut tissues for single-cell atlasing studies

In droplet-based single-cell RNA-sequencing (scRNA-seq) experiments, cells, along with some of their surrounding buffer and ambient material, are encapsulated into droplets for mRNA capture and barcoding. This protocol details the steps for human gut tissue dissociation using cold active protease, a...

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Detalles Bibliográficos
Autores principales: Simmons, Alan J., Lau, Ken S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307676/
https://www.ncbi.nlm.nih.gov/pubmed/35880121
http://dx.doi.org/10.1016/j.xpro.2022.101570

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307676/
https://www.ncbi.nlm.nih.gov/pubmed/35880121
http://dx.doi.org/10.1016/j.xpro.2022.101570

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