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Dissociation and inDrops microfluidic encapsulation of human gut tissues for single-cell atlasing studies
In droplet-based single-cell RNA-sequencing (scRNA-seq) experiments, cells, along with some of their surrounding buffer and ambient material, are encapsulated into droplets for mRNA capture and barcoding. This protocol details the steps for human gut tissue dissociation using cold active protease, a...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307676/ https://www.ncbi.nlm.nih.gov/pubmed/35880121 http://dx.doi.org/10.1016/j.xpro.2022.101570 |