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Protocol for germ lineage differentiation of primed human pluripotent stem cells using chemically defined, nutrient-balanced media
Metabolism regulates cell fates during early mammalian cell differentiation. This protocol describes the steps for directed differentiation of primed human pluripotent stem cells (hPSCs) into the three primary germ lineages—ectoderm, endoderm, and mesoderm—using a chemically defined nutrient-balance...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307681/ https://www.ncbi.nlm.nih.gov/pubmed/35880122 http://dx.doi.org/10.1016/j.xpro.2022.101568 |