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Protocol for germ lineage differentiation of primed human pluripotent stem cells using chemically defined, nutrient-balanced media

Metabolism regulates cell fates during early mammalian cell differentiation. This protocol describes the steps for directed differentiation of primed human pluripotent stem cells (hPSCs) into the three primary germ lineages—ectoderm, endoderm, and mesoderm—using a chemically defined nutrient-balance...

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Detalles Bibliográficos
Autores principales:	Lu, Vivian, Doan, Mary T., Roy, Irena J., Torres, Alejandro, Teitell, Michael A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307681/ https://www.ncbi.nlm.nih.gov/pubmed/35880122 http://dx.doi.org/10.1016/j.xpro.2022.101568

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