Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices

Soft fibrin gels are used to select tumorigenic cells and regulate the stemness and metastasis of colorectal cancer cells via mechanotransduction. This protocol details steps to produce two-dimensional (2D) and three-dimensional (3D) extracellular matrices for substrate rigidity manipulation and tum...

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Autores principales: Zhang, Juan, Ha, Wentao, Chang, Yuhan, Ge, Yutong, Wei, Xiaowei, Tan, Youhua, Jia, Qiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307683/
https://www.ncbi.nlm.nih.gov/pubmed/35880125
http://dx.doi.org/10.1016/j.xpro.2022.101577
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author Zhang, Juan
Ha, Wentao
Chang, Yuhan
Ge, Yutong
Wei, Xiaowei
Tan, Youhua
Jia, Qiong
author_facet Zhang, Juan
Ha, Wentao
Chang, Yuhan
Ge, Yutong
Wei, Xiaowei
Tan, Youhua
Jia, Qiong
author_sort Zhang, Juan
collection PubMed
description Soft fibrin gels are used to select tumorigenic cells and regulate the stemness and metastasis of colorectal cancer cells via mechanotransduction. This protocol details steps to produce two-dimensional (2D) and three-dimensional (3D) extracellular matrices for substrate rigidity manipulation and tumorigenic cell selection. We also describe how it can be applied to tumor mechanotransductive research by colony growth monitoring and cell isolation. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022).
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spelling pubmed-93076832022-07-24 Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices Zhang, Juan Ha, Wentao Chang, Yuhan Ge, Yutong Wei, Xiaowei Tan, Youhua Jia, Qiong STAR Protoc Protocol Soft fibrin gels are used to select tumorigenic cells and regulate the stemness and metastasis of colorectal cancer cells via mechanotransduction. This protocol details steps to produce two-dimensional (2D) and three-dimensional (3D) extracellular matrices for substrate rigidity manipulation and tumorigenic cell selection. We also describe how it can be applied to tumor mechanotransductive research by colony growth monitoring and cell isolation. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022). Elsevier 2022-07-19 /pmc/articles/PMC9307683/ /pubmed/35880125 http://dx.doi.org/10.1016/j.xpro.2022.101577 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhang, Juan
Ha, Wentao
Chang, Yuhan
Ge, Yutong
Wei, Xiaowei
Tan, Youhua
Jia, Qiong
Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices
title Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices
title_full Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices
title_fullStr Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices
title_full_unstemmed Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices
title_short Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices
title_sort tumorigenic cell selection and substrate rigidity manipulation using 2d and 3d extracellular matrices
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307683/
https://www.ncbi.nlm.nih.gov/pubmed/35880125
http://dx.doi.org/10.1016/j.xpro.2022.101577
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