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Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices
Soft fibrin gels are used to select tumorigenic cells and regulate the stemness and metastasis of colorectal cancer cells via mechanotransduction. This protocol details steps to produce two-dimensional (2D) and three-dimensional (3D) extracellular matrices for substrate rigidity manipulation and tum...
Autores principales: | Zhang, Juan, Ha, Wentao, Chang, Yuhan, Ge, Yutong, Wei, Xiaowei, Tan, Youhua, Jia, Qiong |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307683/ https://www.ncbi.nlm.nih.gov/pubmed/35880125 http://dx.doi.org/10.1016/j.xpro.2022.101577 |
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