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Competitive SNP-LAMP probes for rapid and robust single-nucleotide polymorphism detection

In this work, we developed a simple and robust assay to rapidly detect SNPs in nucleic acid samples. Our approach combines loop-mediated isothermal amplification (LAMP)-based target amplification with fluorescent probes to detect SNPs with high specificity. A competitive “sink” strand preferentially...

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Detalles Bibliográficos
Autores principales: Hyman, Leland B., Christopher, Clare R., Romero, Philip A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308130/
https://www.ncbi.nlm.nih.gov/pubmed/35880021
http://dx.doi.org/10.1016/j.crmeth.2022.100242