Learning what not to select for in antibody drug discovery

Identifying antibodies with high affinity and target specificity is crucial for drug discovery and development; however, filtering out antibody candidates with nonspecific or polyspecific binding profiles is also important. In this issue of Cell Reports Methods, Saksena et al. report a computational counterselection method combining deep sequencing and machine learning for identifying nonspecific antibody candidates and demonstrate that it has advantages over more established molecular counterselection methods.