A DNA-Free Editing Platform for Genetic Screens in Soybean via CRISPR/Cas9 Ribonucleoprotein Delivery

CRISPR/Cas9-based ribonucleoprotein (RNP)-mediated system has the property of minimizing the effects related to the unwanted introduction of vector DNA and random integration of recombinant DNA. Here, we describe a platform based on the direct delivery of Cas9 RNPs to soybean protoplasts for genetic...

Descripción completa

Detalles Bibliográficos
Autores principales: Subburaj, Saminathan, Zanatta, Caroline Bedin, Nunn, Jennifer A. L., Hoepers, Aline Martins, Nodari, Rubens Onofre, Agapito-Tenfen, Sarah Zanon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315425/
https://www.ncbi.nlm.nih.gov/pubmed/35903231
http://dx.doi.org/10.3389/fpls.2022.939997

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315425/
https://www.ncbi.nlm.nih.gov/pubmed/35903231
http://dx.doi.org/10.3389/fpls.2022.939997

Ejemplares similares