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A DNA-Free Editing Platform for Genetic Screens in Soybean via CRISPR/Cas9 Ribonucleoprotein Delivery

CRISPR/Cas9-based ribonucleoprotein (RNP)-mediated system has the property of minimizing the effects related to the unwanted introduction of vector DNA and random integration of recombinant DNA. Here, we describe a platform based on the direct delivery of Cas9 RNPs to soybean protoplasts for genetic...

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Detalles Bibliográficos
Autores principales:	Subburaj, Saminathan, Zanatta, Caroline Bedin, Nunn, Jennifer A. L., Hoepers, Aline Martins, Nodari, Rubens Onofre, Agapito-Tenfen, Sarah Zanon
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2022
Materias:	Plant Science
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315425/ https://www.ncbi.nlm.nih.gov/pubmed/35903231 http://dx.doi.org/10.3389/fpls.2022.939997

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