miR-10 and Its Negative Correlation with Serum IL-35 Concentration and Positive Correlation with STAT5a Expression in Patients with Rheumatoid Arthritis

Circulating free-cell miRNAs are increasingly important as potential non-invasive biomarkers due to the easy accessibility of clinical materials. Moreover, their epigenetic role may provide insight into the mechanisms of pathogenesis. Nevertheless, these aspects are mostly studied in the area of onc...

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Autores principales: Paradowska-Gorycka, Agnieszka, Wajda, Anna, Rzeszotarska, Ewa, Kmiolek, Tomasz, Stypinska, Barbara, Dudek, Ewa, Romanowska-Prochnicka, Katarzyna, Syrowka, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317037/
https://www.ncbi.nlm.nih.gov/pubmed/35887269
http://dx.doi.org/10.3390/ijms23147925
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author Paradowska-Gorycka, Agnieszka
Wajda, Anna
Rzeszotarska, Ewa
Kmiolek, Tomasz
Stypinska, Barbara
Dudek, Ewa
Romanowska-Prochnicka, Katarzyna
Syrowka, Piotr
author_facet Paradowska-Gorycka, Agnieszka
Wajda, Anna
Rzeszotarska, Ewa
Kmiolek, Tomasz
Stypinska, Barbara
Dudek, Ewa
Romanowska-Prochnicka, Katarzyna
Syrowka, Piotr
author_sort Paradowska-Gorycka, Agnieszka
collection PubMed
description Circulating free-cell miRNAs are increasingly important as potential non-invasive biomarkers due to the easy accessibility of clinical materials. Moreover, their epigenetic role may provide insight into the mechanisms of pathogenesis. Nevertheless, these aspects are mostly studied in the area of oncological diseases. Therefore, this research aimed to find the potential association of selected miRNAs in serum with the expression of Th17/Treg transcription factors and clinical features in RA patients. Accordingly, experiments was conducted on rheumatoid arthritis (RA), osteoarthritis (OA) and healthy subjects (HC). Analysis of miRNAs level in serum was performed using LNA miRNA PCR assays. mir-10 was detected only in RA patients. Furthermore, its expression was correlated with IL-35 serum concentration and the mRNA level of STAT5a in whole blood in RA. Additionally, a tendency of the raised level of miR-10 was noted in RA patients with high activity disease. miR-326 was significantly upregulated in RA patients with rheumatoid factor presence. In HC the correlation between miR-26 and IL-21 serum levels and expression of SMAD3 have been found. In OA patients, correlations between miR-126 and HIF1 expression and between miR-146 and RORc have been noted. The differential association of transcription factor expression with serum miRNA levels may be important in the diagnosis and progression of RA and OA.
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spelling pubmed-93170372022-07-27 miR-10 and Its Negative Correlation with Serum IL-35 Concentration and Positive Correlation with STAT5a Expression in Patients with Rheumatoid Arthritis Paradowska-Gorycka, Agnieszka Wajda, Anna Rzeszotarska, Ewa Kmiolek, Tomasz Stypinska, Barbara Dudek, Ewa Romanowska-Prochnicka, Katarzyna Syrowka, Piotr Int J Mol Sci Article Circulating free-cell miRNAs are increasingly important as potential non-invasive biomarkers due to the easy accessibility of clinical materials. Moreover, their epigenetic role may provide insight into the mechanisms of pathogenesis. Nevertheless, these aspects are mostly studied in the area of oncological diseases. Therefore, this research aimed to find the potential association of selected miRNAs in serum with the expression of Th17/Treg transcription factors and clinical features in RA patients. Accordingly, experiments was conducted on rheumatoid arthritis (RA), osteoarthritis (OA) and healthy subjects (HC). Analysis of miRNAs level in serum was performed using LNA miRNA PCR assays. mir-10 was detected only in RA patients. Furthermore, its expression was correlated with IL-35 serum concentration and the mRNA level of STAT5a in whole blood in RA. Additionally, a tendency of the raised level of miR-10 was noted in RA patients with high activity disease. miR-326 was significantly upregulated in RA patients with rheumatoid factor presence. In HC the correlation between miR-26 and IL-21 serum levels and expression of SMAD3 have been found. In OA patients, correlations between miR-126 and HIF1 expression and between miR-146 and RORc have been noted. The differential association of transcription factor expression with serum miRNA levels may be important in the diagnosis and progression of RA and OA. MDPI 2022-07-18 /pmc/articles/PMC9317037/ /pubmed/35887269 http://dx.doi.org/10.3390/ijms23147925 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Paradowska-Gorycka, Agnieszka
Wajda, Anna
Rzeszotarska, Ewa
Kmiolek, Tomasz
Stypinska, Barbara
Dudek, Ewa
Romanowska-Prochnicka, Katarzyna
Syrowka, Piotr
miR-10 and Its Negative Correlation with Serum IL-35 Concentration and Positive Correlation with STAT5a Expression in Patients with Rheumatoid Arthritis
title miR-10 and Its Negative Correlation with Serum IL-35 Concentration and Positive Correlation with STAT5a Expression in Patients with Rheumatoid Arthritis
title_full miR-10 and Its Negative Correlation with Serum IL-35 Concentration and Positive Correlation with STAT5a Expression in Patients with Rheumatoid Arthritis
title_fullStr miR-10 and Its Negative Correlation with Serum IL-35 Concentration and Positive Correlation with STAT5a Expression in Patients with Rheumatoid Arthritis
title_full_unstemmed miR-10 and Its Negative Correlation with Serum IL-35 Concentration and Positive Correlation with STAT5a Expression in Patients with Rheumatoid Arthritis
title_short miR-10 and Its Negative Correlation with Serum IL-35 Concentration and Positive Correlation with STAT5a Expression in Patients with Rheumatoid Arthritis
title_sort mir-10 and its negative correlation with serum il-35 concentration and positive correlation with stat5a expression in patients with rheumatoid arthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317037/
https://www.ncbi.nlm.nih.gov/pubmed/35887269
http://dx.doi.org/10.3390/ijms23147925
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