The Matrix Effect in the RT-PCR Detection of SARS-CoV-2 Using Saliva without RNA Extraction

The present work focuses on the detection of SARS-CoV-2 in saliva, contributing to understanding the inhibition effect of the matrix and its influence on the results. Detection of viral genes ORF1ab, N, and E was performed by RT-PCR using saliva directly in the reaction without RNA extraction. Diffe...

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Detalles Bibliográficos
Autores principales: Morais, Orlando, Alves, Manuel Rui, Ramos, Carla, Ferreira, Fernando, Fernandes, Paulo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318260/
https://www.ncbi.nlm.nih.gov/pubmed/35885453
http://dx.doi.org/10.3390/diagnostics12071547

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318260/
https://www.ncbi.nlm.nih.gov/pubmed/35885453
http://dx.doi.org/10.3390/diagnostics12071547

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