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Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis

The epithelial-mesenchymal transition (EMT), a process in which epithelial cells undergo a series of biochemical changes to acquire a mesenchymal phenotype, has been linked to tumor metastasis. Here, we present a novel strategy for knocking out the EMT-related Cdh2 gene, which encodes N-cadherin thr...

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Autores principales: Gao, Ruru, Luo, Qiong, Li, Yang, Song, Liming, Cai, Junnan (Stephen), Xiong, Ying, Yan, Fei, Liu, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319454/
https://www.ncbi.nlm.nih.gov/pubmed/35890278
http://dx.doi.org/10.3390/pharmaceutics14071382
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author Gao, Ruru
Luo, Qiong
Li, Yang
Song, Liming
Cai, Junnan (Stephen)
Xiong, Ying
Yan, Fei
Liu, Jianhua
author_facet Gao, Ruru
Luo, Qiong
Li, Yang
Song, Liming
Cai, Junnan (Stephen)
Xiong, Ying
Yan, Fei
Liu, Jianhua
author_sort Gao, Ruru
collection PubMed
description The epithelial-mesenchymal transition (EMT), a process in which epithelial cells undergo a series of biochemical changes to acquire a mesenchymal phenotype, has been linked to tumor metastasis. Here, we present a novel strategy for knocking out the EMT-related Cdh2 gene, which encodes N-cadherin through CRISPR/Cas9-mediated gene editing by an ultrasound combined with biosynthetic nanobubbles (Gas Vesicles, GVs). Polyethyleneimine were employed as a gene delivery vector to deliver sgRNA into 4T1 cells that stably express the Cas9 protein, resulting in the stable Cdh2 gene- knockout cell lines. The Western blotting assay confirmed the absence of an N-cadherin protein in these Cdh2 gene-knockout 4T1 cell lines. Significantly reduced tumor cell migration was observed in the Cdh2 gene-knockout 4T1 cells in comparison with the wild-type cells. Our study demonstrated that an ultrasound combined with GVs could effectively mediate CRISPR/Cas9 gene editing of a Cdh2 gene to inhibit tumor invasion and metastasis.
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spelling pubmed-93194542022-07-27 Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis Gao, Ruru Luo, Qiong Li, Yang Song, Liming Cai, Junnan (Stephen) Xiong, Ying Yan, Fei Liu, Jianhua Pharmaceutics Article The epithelial-mesenchymal transition (EMT), a process in which epithelial cells undergo a series of biochemical changes to acquire a mesenchymal phenotype, has been linked to tumor metastasis. Here, we present a novel strategy for knocking out the EMT-related Cdh2 gene, which encodes N-cadherin through CRISPR/Cas9-mediated gene editing by an ultrasound combined with biosynthetic nanobubbles (Gas Vesicles, GVs). Polyethyleneimine were employed as a gene delivery vector to deliver sgRNA into 4T1 cells that stably express the Cas9 protein, resulting in the stable Cdh2 gene- knockout cell lines. The Western blotting assay confirmed the absence of an N-cadherin protein in these Cdh2 gene-knockout 4T1 cell lines. Significantly reduced tumor cell migration was observed in the Cdh2 gene-knockout 4T1 cells in comparison with the wild-type cells. Our study demonstrated that an ultrasound combined with GVs could effectively mediate CRISPR/Cas9 gene editing of a Cdh2 gene to inhibit tumor invasion and metastasis. MDPI 2022-06-30 /pmc/articles/PMC9319454/ /pubmed/35890278 http://dx.doi.org/10.3390/pharmaceutics14071382 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gao, Ruru
Luo, Qiong
Li, Yang
Song, Liming
Cai, Junnan (Stephen)
Xiong, Ying
Yan, Fei
Liu, Jianhua
Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis
title Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis
title_full Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis
title_fullStr Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis
title_full_unstemmed Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis
title_short Biosynthetic Nanobubble-Mediated CRISPR/Cas9 Gene Editing of Cdh2 Inhibits Breast Cancer Metastasis
title_sort biosynthetic nanobubble-mediated crispr/cas9 gene editing of cdh2 inhibits breast cancer metastasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319454/
https://www.ncbi.nlm.nih.gov/pubmed/35890278
http://dx.doi.org/10.3390/pharmaceutics14071382
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