Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering

CRISPR–Cas9 is the state-of-the-art programmable genome-editing tool widely used in many areas. For safe therapeutic applications in clinical medicine, its off-target effect must be dramatically minimized. In recent years, extensive studies have been conducted to improve the gene-editing specificity...

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Detalles Bibliográficos
Autores principales: Huang, Xiaoqiang, Yang, Dongshan, Zhang, Jifeng, Xu, Jie, Chen, Y. Eugene
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319960/
https://www.ncbi.nlm.nih.gov/pubmed/35883629
http://dx.doi.org/10.3390/cells11142186
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author Huang, Xiaoqiang
Yang, Dongshan
Zhang, Jifeng
Xu, Jie
Chen, Y. Eugene
author_facet Huang, Xiaoqiang
Yang, Dongshan
Zhang, Jifeng
Xu, Jie
Chen, Y. Eugene
author_sort Huang, Xiaoqiang
collection PubMed
description CRISPR–Cas9 is the state-of-the-art programmable genome-editing tool widely used in many areas. For safe therapeutic applications in clinical medicine, its off-target effect must be dramatically minimized. In recent years, extensive studies have been conducted to improve the gene-editing specificity of the most popular CRISPR–Cas9 nucleases using different strategies. In this review, we summarize and discuss these strategies and achievements, with a major focus on improving the gene-editing specificity through Cas9 protein engineering.
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spelling pubmed-93199602022-07-27 Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering Huang, Xiaoqiang Yang, Dongshan Zhang, Jifeng Xu, Jie Chen, Y. Eugene Cells Review CRISPR–Cas9 is the state-of-the-art programmable genome-editing tool widely used in many areas. For safe therapeutic applications in clinical medicine, its off-target effect must be dramatically minimized. In recent years, extensive studies have been conducted to improve the gene-editing specificity of the most popular CRISPR–Cas9 nucleases using different strategies. In this review, we summarize and discuss these strategies and achievements, with a major focus on improving the gene-editing specificity through Cas9 protein engineering. MDPI 2022-07-13 /pmc/articles/PMC9319960/ /pubmed/35883629 http://dx.doi.org/10.3390/cells11142186 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Huang, Xiaoqiang
Yang, Dongshan
Zhang, Jifeng
Xu, Jie
Chen, Y. Eugene
Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering
title Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering
title_full Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering
title_fullStr Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering
title_full_unstemmed Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering
title_short Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering
title_sort recent advances in improving gene-editing specificity through crispr–cas9 nuclease engineering
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319960/
https://www.ncbi.nlm.nih.gov/pubmed/35883629
http://dx.doi.org/10.3390/cells11142186
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