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Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction

In the present research, an orange extract (OE) was obtained and encapsulated in a zein matrix for its subsequent physicochemical characterization and evaluation of its antioxidant capacity. The OE consists of phenolic compounds and flavonoids extracted from orange peel (Citrus sinensis) by ultrasou...

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Autores principales: Luque-Alcaraz, Ana G., Velazquez-Antillón, Miranda, Hernández-Téllez, Cynthia N., Graciano-Verdugo, Abril Z., García-Flores, Nadia, Iriqui-Razcón, Jorge L., Silvas-García, María Irene, Zazueta-Raynaud, Aldo, Moreno-Vásquez, María J., Hernández-Abril, Pedro A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320882/
https://www.ncbi.nlm.nih.gov/pubmed/35888305
http://dx.doi.org/10.3390/ma15144838
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author Luque-Alcaraz, Ana G.
Velazquez-Antillón, Miranda
Hernández-Téllez, Cynthia N.
Graciano-Verdugo, Abril Z.
García-Flores, Nadia
Iriqui-Razcón, Jorge L.
Silvas-García, María Irene
Zazueta-Raynaud, Aldo
Moreno-Vásquez, María J.
Hernández-Abril, Pedro A.
author_facet Luque-Alcaraz, Ana G.
Velazquez-Antillón, Miranda
Hernández-Téllez, Cynthia N.
Graciano-Verdugo, Abril Z.
García-Flores, Nadia
Iriqui-Razcón, Jorge L.
Silvas-García, María Irene
Zazueta-Raynaud, Aldo
Moreno-Vásquez, María J.
Hernández-Abril, Pedro A.
author_sort Luque-Alcaraz, Ana G.
collection PubMed
description In the present research, an orange extract (OE) was obtained and encapsulated in a zein matrix for its subsequent physicochemical characterization and evaluation of its antioxidant capacity. The OE consists of phenolic compounds and flavonoids extracted from orange peel (Citrus sinensis) by ultrasound-assisted extraction (UAE). The results obtained by dynamic light scattering (DLS) and scanning electron microscopy (SEM) indicated that zein nanoparticles with orange extract (NpZOE) presented a nanometric size and spherical shape, presenting a hydrodynamic diameter of 159.26 ± 5.96 nm. Furthermore, ζ-potential evolution and Fourier transform infrared spectroscopy (FTIR) techniques were used to evaluate the interaction between zein and OE. Regarding antioxidant activity, ABTS and DPPH assays indicated no significant differences at high concentrations of orange peel extract and NpZOE; however, NpZOE was more effective at low concentrations. Although this indicates that ultrasonication as an extraction method effectively obtains the phenolic compounds present in orange peels, the nanoprecipitation method under the conditions used allowed us to obtain particles in the nanometric range with positive ζ-potential. On the other hand, the antioxidant capacity analysis indicated a high antioxidant capacity of both OE and the NpZOE. This study presents the possibility of obtaining orange extracts by ultrasound and coupling them to zein-based nanoparticulate systems to be applied as biomedical materials functionalized with antioxidant substances of pharmaceutical utility.
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spelling pubmed-93208822022-07-27 Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction Luque-Alcaraz, Ana G. Velazquez-Antillón, Miranda Hernández-Téllez, Cynthia N. Graciano-Verdugo, Abril Z. García-Flores, Nadia Iriqui-Razcón, Jorge L. Silvas-García, María Irene Zazueta-Raynaud, Aldo Moreno-Vásquez, María J. Hernández-Abril, Pedro A. Materials (Basel) Article In the present research, an orange extract (OE) was obtained and encapsulated in a zein matrix for its subsequent physicochemical characterization and evaluation of its antioxidant capacity. The OE consists of phenolic compounds and flavonoids extracted from orange peel (Citrus sinensis) by ultrasound-assisted extraction (UAE). The results obtained by dynamic light scattering (DLS) and scanning electron microscopy (SEM) indicated that zein nanoparticles with orange extract (NpZOE) presented a nanometric size and spherical shape, presenting a hydrodynamic diameter of 159.26 ± 5.96 nm. Furthermore, ζ-potential evolution and Fourier transform infrared spectroscopy (FTIR) techniques were used to evaluate the interaction between zein and OE. Regarding antioxidant activity, ABTS and DPPH assays indicated no significant differences at high concentrations of orange peel extract and NpZOE; however, NpZOE was more effective at low concentrations. Although this indicates that ultrasonication as an extraction method effectively obtains the phenolic compounds present in orange peels, the nanoprecipitation method under the conditions used allowed us to obtain particles in the nanometric range with positive ζ-potential. On the other hand, the antioxidant capacity analysis indicated a high antioxidant capacity of both OE and the NpZOE. This study presents the possibility of obtaining orange extracts by ultrasound and coupling them to zein-based nanoparticulate systems to be applied as biomedical materials functionalized with antioxidant substances of pharmaceutical utility. MDPI 2022-07-12 /pmc/articles/PMC9320882/ /pubmed/35888305 http://dx.doi.org/10.3390/ma15144838 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luque-Alcaraz, Ana G.
Velazquez-Antillón, Miranda
Hernández-Téllez, Cynthia N.
Graciano-Verdugo, Abril Z.
García-Flores, Nadia
Iriqui-Razcón, Jorge L.
Silvas-García, María Irene
Zazueta-Raynaud, Aldo
Moreno-Vásquez, María J.
Hernández-Abril, Pedro A.
Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction
title Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction
title_full Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction
title_fullStr Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction
title_full_unstemmed Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction
title_short Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction
title_sort antioxidant effect of nanoparticles composed of zein and orange (citrus sinensis) extract obtained by ultrasound-assisted extraction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320882/
https://www.ncbi.nlm.nih.gov/pubmed/35888305
http://dx.doi.org/10.3390/ma15144838
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