Screening of Genes Related to Sex Determination and Differentiation in Mandarin Fish (Siniperca chuatsi)

Mandarin fish has an XX/XY sex-determination system. The female mandarin fish is typically larger than the male. Sex identification and the discovery of genes related to sex determination in mandarin fish have important theoretical significance in the elucidation of the regulation and evolutionary m...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Cheng, Chen, Liangming, Huang, Rong, Gui, Bin, Li, Yangyu, Li, Yangyang, Li, Yongming, Liao, Lanjie, Zhu, Zuoyan, Wang, Yaping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321114/
https://www.ncbi.nlm.nih.gov/pubmed/35887035
http://dx.doi.org/10.3390/ijms23147692
_version_ 1784755959153819648
author Yang, Cheng
Chen, Liangming
Huang, Rong
Gui, Bin
Li, Yangyu
Li, Yangyang
Li, Yongming
Liao, Lanjie
Zhu, Zuoyan
Wang, Yaping
author_facet Yang, Cheng
Chen, Liangming
Huang, Rong
Gui, Bin
Li, Yangyu
Li, Yangyang
Li, Yongming
Liao, Lanjie
Zhu, Zuoyan
Wang, Yaping
author_sort Yang, Cheng
collection PubMed
description Mandarin fish has an XX/XY sex-determination system. The female mandarin fish is typically larger than the male. Sex identification and the discovery of genes related to sex determination in mandarin fish have important theoretical significance in the elucidation of the regulation and evolutionary mechanism of animal reproductive development. In this study, the chromosome-level genome of a female mandarin fish was assembled, and we found that LG24 of the genome was an X chromosome. A total of 61 genes on the X chromosome showed sex-biased expression. Only six gonadal genes (LG24G00426, LG24G003280, LG24G003300, LG24G003730, LG24G004200, and LG24G004770) were expressed in the testes, and the expression of the other gene LG24G003870 isoform 1 in the ovaries was significantly higher than that in the testes (p < 0.01). Five (except LG24G003280 and LG24G003300) of the seven aforementioned genes were expressed at the embryonic development stage, suggesting their involvement in early sex determination. The expression of LG24G004770 (encoding HS6ST 3-B-like) was also significantly higher in female muscles than in male muscles (p < 0.01), indicating other functions related to female growth. ZP3 encoded by LG24G003870 isoform 1 increased the C-terminal transmembrane domain, compared with that encoded by other fish zp3 isoforms, indicating their different functions in sex determination or differentiation. This study provides a foundation for the identification of sex-determining genes in mandarin fish.
format Online
Article
Text
id pubmed-9321114
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93211142022-07-27 Screening of Genes Related to Sex Determination and Differentiation in Mandarin Fish (Siniperca chuatsi) Yang, Cheng Chen, Liangming Huang, Rong Gui, Bin Li, Yangyu Li, Yangyang Li, Yongming Liao, Lanjie Zhu, Zuoyan Wang, Yaping Int J Mol Sci Brief Report Mandarin fish has an XX/XY sex-determination system. The female mandarin fish is typically larger than the male. Sex identification and the discovery of genes related to sex determination in mandarin fish have important theoretical significance in the elucidation of the regulation and evolutionary mechanism of animal reproductive development. In this study, the chromosome-level genome of a female mandarin fish was assembled, and we found that LG24 of the genome was an X chromosome. A total of 61 genes on the X chromosome showed sex-biased expression. Only six gonadal genes (LG24G00426, LG24G003280, LG24G003300, LG24G003730, LG24G004200, and LG24G004770) were expressed in the testes, and the expression of the other gene LG24G003870 isoform 1 in the ovaries was significantly higher than that in the testes (p < 0.01). Five (except LG24G003280 and LG24G003300) of the seven aforementioned genes were expressed at the embryonic development stage, suggesting their involvement in early sex determination. The expression of LG24G004770 (encoding HS6ST 3-B-like) was also significantly higher in female muscles than in male muscles (p < 0.01), indicating other functions related to female growth. ZP3 encoded by LG24G003870 isoform 1 increased the C-terminal transmembrane domain, compared with that encoded by other fish zp3 isoforms, indicating their different functions in sex determination or differentiation. This study provides a foundation for the identification of sex-determining genes in mandarin fish. MDPI 2022-07-12 /pmc/articles/PMC9321114/ /pubmed/35887035 http://dx.doi.org/10.3390/ijms23147692 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Yang, Cheng
Chen, Liangming
Huang, Rong
Gui, Bin
Li, Yangyu
Li, Yangyang
Li, Yongming
Liao, Lanjie
Zhu, Zuoyan
Wang, Yaping
Screening of Genes Related to Sex Determination and Differentiation in Mandarin Fish (Siniperca chuatsi)
title Screening of Genes Related to Sex Determination and Differentiation in Mandarin Fish (Siniperca chuatsi)
title_full Screening of Genes Related to Sex Determination and Differentiation in Mandarin Fish (Siniperca chuatsi)
title_fullStr Screening of Genes Related to Sex Determination and Differentiation in Mandarin Fish (Siniperca chuatsi)
title_full_unstemmed Screening of Genes Related to Sex Determination and Differentiation in Mandarin Fish (Siniperca chuatsi)
title_short Screening of Genes Related to Sex Determination and Differentiation in Mandarin Fish (Siniperca chuatsi)
title_sort screening of genes related to sex determination and differentiation in mandarin fish (siniperca chuatsi)
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321114/
https://www.ncbi.nlm.nih.gov/pubmed/35887035
http://dx.doi.org/10.3390/ijms23147692
work_keys_str_mv AT yangcheng screeningofgenesrelatedtosexdeterminationanddifferentiationinmandarinfishsinipercachuatsi
AT chenliangming screeningofgenesrelatedtosexdeterminationanddifferentiationinmandarinfishsinipercachuatsi
AT huangrong screeningofgenesrelatedtosexdeterminationanddifferentiationinmandarinfishsinipercachuatsi
AT guibin screeningofgenesrelatedtosexdeterminationanddifferentiationinmandarinfishsinipercachuatsi
AT liyangyu screeningofgenesrelatedtosexdeterminationanddifferentiationinmandarinfishsinipercachuatsi
AT liyangyang screeningofgenesrelatedtosexdeterminationanddifferentiationinmandarinfishsinipercachuatsi
AT liyongming screeningofgenesrelatedtosexdeterminationanddifferentiationinmandarinfishsinipercachuatsi
AT liaolanjie screeningofgenesrelatedtosexdeterminationanddifferentiationinmandarinfishsinipercachuatsi
AT zhuzuoyan screeningofgenesrelatedtosexdeterminationanddifferentiationinmandarinfishsinipercachuatsi
AT wangyaping screeningofgenesrelatedtosexdeterminationanddifferentiationinmandarinfishsinipercachuatsi