Determination of ractopamine and salbutamol in pig hair by liquid chromatography tandem mass spectrometry

A liquid chromatography tandem mass spectrometric method was developed for the determination of two β-agonists (ractopamine and salbutamol) in pig hair samples. An isotope of ractopamine-d5 or salbutamol-d6 as an internal standard was used to carry out quantitative analysis. Concentrated sodium hydr...

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Autores principales: Chang, Kai-Chun, Chang, Yu-Ting, Tsai, Chin-En
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322239/
https://www.ncbi.nlm.nih.gov/pubmed/29567243
http://dx.doi.org/10.1016/j.jfda.2017.09.005
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author Chang, Kai-Chun
Chang, Yu-Ting
Tsai, Chin-En
author_facet Chang, Kai-Chun
Chang, Yu-Ting
Tsai, Chin-En
author_sort Chang, Kai-Chun
collection PubMed
description A liquid chromatography tandem mass spectrometric method was developed for the determination of two β-agonists (ractopamine and salbutamol) in pig hair samples. An isotope of ractopamine-d5 or salbutamol-d6 as an internal standard was used to carry out quantitative analysis. Concentrated sodium hydroxide was used to pretreat hair samples and then purified by the solid phase extraction (SPE) procedure. The extracted solution was evaporated and reconstituted for injection in the instrument with electrospray ionization (ESI) operating in a positive multiple-reaction-monitoring (MRM) mode. Ractopamine and salbutamol separation were performed on C18 analytical column under gradient condition. The internal standard calibration curve was linear in the range of concentration from 0.5 to 100 ng mL(−1) (R(2) > 0.995). Recoveries of this method estimated at three spiked concentrations of 100, 250 and 500 ng mL(−1) in pig hair samples, were 79–82% for ractopamine and 77–96% for salbutamol. The corresponding inter-day and intra-day precisions expressed as relative standard deviation (RSD %) were 3.8–6.4% and 3.8–8.6%, respectively. The analytical time for one sample was 8 min. The detection limit of this method was 0.6 and 8.3 ng mL(−1) for ractopamine and salbutamol, respectively. This developed method can be applied for monitoring the use of the β-agonists salbutamol and ractopamine in swine feed incurred pig hair.
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spelling pubmed-93222392022-08-09 Determination of ractopamine and salbutamol in pig hair by liquid chromatography tandem mass spectrometry Chang, Kai-Chun Chang, Yu-Ting Tsai, Chin-En J Food Drug Anal Original Article A liquid chromatography tandem mass spectrometric method was developed for the determination of two β-agonists (ractopamine and salbutamol) in pig hair samples. An isotope of ractopamine-d5 or salbutamol-d6 as an internal standard was used to carry out quantitative analysis. Concentrated sodium hydroxide was used to pretreat hair samples and then purified by the solid phase extraction (SPE) procedure. The extracted solution was evaporated and reconstituted for injection in the instrument with electrospray ionization (ESI) operating in a positive multiple-reaction-monitoring (MRM) mode. Ractopamine and salbutamol separation were performed on C18 analytical column under gradient condition. The internal standard calibration curve was linear in the range of concentration from 0.5 to 100 ng mL(−1) (R(2) > 0.995). Recoveries of this method estimated at three spiked concentrations of 100, 250 and 500 ng mL(−1) in pig hair samples, were 79–82% for ractopamine and 77–96% for salbutamol. The corresponding inter-day and intra-day precisions expressed as relative standard deviation (RSD %) were 3.8–6.4% and 3.8–8.6%, respectively. The analytical time for one sample was 8 min. The detection limit of this method was 0.6 and 8.3 ng mL(−1) for ractopamine and salbutamol, respectively. This developed method can be applied for monitoring the use of the β-agonists salbutamol and ractopamine in swine feed incurred pig hair. Taiwan Food and Drug Administration 2017-11-12 /pmc/articles/PMC9322239/ /pubmed/29567243 http://dx.doi.org/10.1016/j.jfda.2017.09.005 Text en © 2018 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Chang, Kai-Chun
Chang, Yu-Ting
Tsai, Chin-En
Determination of ractopamine and salbutamol in pig hair by liquid chromatography tandem mass spectrometry
title Determination of ractopamine and salbutamol in pig hair by liquid chromatography tandem mass spectrometry
title_full Determination of ractopamine and salbutamol in pig hair by liquid chromatography tandem mass spectrometry
title_fullStr Determination of ractopamine and salbutamol in pig hair by liquid chromatography tandem mass spectrometry
title_full_unstemmed Determination of ractopamine and salbutamol in pig hair by liquid chromatography tandem mass spectrometry
title_short Determination of ractopamine and salbutamol in pig hair by liquid chromatography tandem mass spectrometry
title_sort determination of ractopamine and salbutamol in pig hair by liquid chromatography tandem mass spectrometry
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322239/
https://www.ncbi.nlm.nih.gov/pubmed/29567243
http://dx.doi.org/10.1016/j.jfda.2017.09.005
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