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Capturing Transitional Pluripotency through Proline Metabolism

In this paper, we summarize the current knowledge of the role of proline metabolism in the control of the identity of Embryonic Stem Cells (ESCs). An imbalance in proline metabolism shifts mouse ESCs toward a stable naïve-to-primed intermediate state of pluripotency. Proline-induced cells (PiCs), al...

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Autores principales: Minchiotti, Gabriella, D’Aniello, Cristina, Fico, Annalisa, De Cesare, Dario, Patriarca, Eduardo Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323356/
https://www.ncbi.nlm.nih.gov/pubmed/35883568
http://dx.doi.org/10.3390/cells11142125
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author Minchiotti, Gabriella
D’Aniello, Cristina
Fico, Annalisa
De Cesare, Dario
Patriarca, Eduardo Jorge
author_facet Minchiotti, Gabriella
D’Aniello, Cristina
Fico, Annalisa
De Cesare, Dario
Patriarca, Eduardo Jorge
author_sort Minchiotti, Gabriella
collection PubMed
description In this paper, we summarize the current knowledge of the role of proline metabolism in the control of the identity of Embryonic Stem Cells (ESCs). An imbalance in proline metabolism shifts mouse ESCs toward a stable naïve-to-primed intermediate state of pluripotency. Proline-induced cells (PiCs), also named primitive ectoderm-like cells (EPLs), are phenotypically metastable, a trait linked to a rapid and reversible relocalization of E-cadherin from the plasma membrane to intracellular membrane compartments. The ESC-to-PiC transition relies on the activation of Erk and Tgfβ/Activin signaling pathways and is associated with extensive remodeling of the transcriptome, metabolome and epigenome. PiCs maintain several properties of naïve pluripotency (teratoma formation, blastocyst colonization and 3D gastruloid development) and acquire a few traits of primed cells (flat-shaped colony morphology, aerobic glycolysis metabolism and competence for primordial germ cell fate). Overall, the molecular and phenotypic features of PiCs resemble those of an early-primed state of pluripotency, providing a robust model to study the role of metabolic perturbations in pluripotency and cell fate decisions.
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spelling pubmed-93233562022-07-27 Capturing Transitional Pluripotency through Proline Metabolism Minchiotti, Gabriella D’Aniello, Cristina Fico, Annalisa De Cesare, Dario Patriarca, Eduardo Jorge Cells Review In this paper, we summarize the current knowledge of the role of proline metabolism in the control of the identity of Embryonic Stem Cells (ESCs). An imbalance in proline metabolism shifts mouse ESCs toward a stable naïve-to-primed intermediate state of pluripotency. Proline-induced cells (PiCs), also named primitive ectoderm-like cells (EPLs), are phenotypically metastable, a trait linked to a rapid and reversible relocalization of E-cadherin from the plasma membrane to intracellular membrane compartments. The ESC-to-PiC transition relies on the activation of Erk and Tgfβ/Activin signaling pathways and is associated with extensive remodeling of the transcriptome, metabolome and epigenome. PiCs maintain several properties of naïve pluripotency (teratoma formation, blastocyst colonization and 3D gastruloid development) and acquire a few traits of primed cells (flat-shaped colony morphology, aerobic glycolysis metabolism and competence for primordial germ cell fate). Overall, the molecular and phenotypic features of PiCs resemble those of an early-primed state of pluripotency, providing a robust model to study the role of metabolic perturbations in pluripotency and cell fate decisions. MDPI 2022-07-06 /pmc/articles/PMC9323356/ /pubmed/35883568 http://dx.doi.org/10.3390/cells11142125 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Minchiotti, Gabriella
D’Aniello, Cristina
Fico, Annalisa
De Cesare, Dario
Patriarca, Eduardo Jorge
Capturing Transitional Pluripotency through Proline Metabolism
title Capturing Transitional Pluripotency through Proline Metabolism
title_full Capturing Transitional Pluripotency through Proline Metabolism
title_fullStr Capturing Transitional Pluripotency through Proline Metabolism
title_full_unstemmed Capturing Transitional Pluripotency through Proline Metabolism
title_short Capturing Transitional Pluripotency through Proline Metabolism
title_sort capturing transitional pluripotency through proline metabolism
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323356/
https://www.ncbi.nlm.nih.gov/pubmed/35883568
http://dx.doi.org/10.3390/cells11142125
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