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Comparative Characterization of Different Molecular Formats of Bispecific Antibodies Targeting EGFR and PD-L1

We generated two IgG1-like bispecific antibodies (BsAbs) with different molecular formats, symmetrical DVD-Ig and asymmetrical knob-in-hole (KIH), targeting the same antigens, EGFR and PD-L1 (designated as anti-EGFR/PD-L1). We performed the physiochemical and biological characterization of these two...

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Autores principales: Mohan, Nishant, Agrawal, Atul, Shen, Yi, Winarski, Katie L., Endo, Yukinori, Dokmanovic, Milos, Schmiel, Deborah, Zheng, Jiwen, Rotstein, David S., Pelosof, Lorraine C., Wu, Wen Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325241/
https://www.ncbi.nlm.nih.gov/pubmed/35890277
http://dx.doi.org/10.3390/pharmaceutics14071381
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author Mohan, Nishant
Agrawal, Atul
Shen, Yi
Winarski, Katie L.
Endo, Yukinori
Dokmanovic, Milos
Schmiel, Deborah
Zheng, Jiwen
Rotstein, David S.
Pelosof, Lorraine C.
Wu, Wen Jin
author_facet Mohan, Nishant
Agrawal, Atul
Shen, Yi
Winarski, Katie L.
Endo, Yukinori
Dokmanovic, Milos
Schmiel, Deborah
Zheng, Jiwen
Rotstein, David S.
Pelosof, Lorraine C.
Wu, Wen Jin
author_sort Mohan, Nishant
collection PubMed
description We generated two IgG1-like bispecific antibodies (BsAbs) with different molecular formats, symmetrical DVD-Ig and asymmetrical knob-in-hole (KIH), targeting the same antigens, EGFR and PD-L1 (designated as anti-EGFR/PD-L1). We performed the physiochemical and biological characterization of these two formats of anti-EGFR/PD-L1 BsAbs and compared some key quality attributes and biological activities of these two formats of BsAbs. Physiochemical binding characterization data demonstrated that both formats bound EGFR and PD-L1. However, the binding affinity of the KIH format was weaker than the DVD-Ig format in Biacore binding assays. In contrast, both DVD-Ig and KIH BsAbs had similar ELISA and cell surface binding activities, comparable to mAbs. Triple-negative breast cancer (TNBC) cells and a xenograft model were used to test the potency of BsAbs and other biological activities. Results showed that anti-EGFR/PD-L1 BsAbs exhibited in vitro and in vivo antitumor proliferation activity, but there was a difference in the potencies of the respective BsAb formats (DVD-Ig and KIH) when different cells or assays were used. This study provides evidence that the potency of the BsAbs targeting the same antigens can be affected by the respective molecular features, and selection of appropriate cell lines and assays is critically important for the assay development and potency testing of BsAbs.
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spelling pubmed-93252412022-07-27 Comparative Characterization of Different Molecular Formats of Bispecific Antibodies Targeting EGFR and PD-L1 Mohan, Nishant Agrawal, Atul Shen, Yi Winarski, Katie L. Endo, Yukinori Dokmanovic, Milos Schmiel, Deborah Zheng, Jiwen Rotstein, David S. Pelosof, Lorraine C. Wu, Wen Jin Pharmaceutics Article We generated two IgG1-like bispecific antibodies (BsAbs) with different molecular formats, symmetrical DVD-Ig and asymmetrical knob-in-hole (KIH), targeting the same antigens, EGFR and PD-L1 (designated as anti-EGFR/PD-L1). We performed the physiochemical and biological characterization of these two formats of anti-EGFR/PD-L1 BsAbs and compared some key quality attributes and biological activities of these two formats of BsAbs. Physiochemical binding characterization data demonstrated that both formats bound EGFR and PD-L1. However, the binding affinity of the KIH format was weaker than the DVD-Ig format in Biacore binding assays. In contrast, both DVD-Ig and KIH BsAbs had similar ELISA and cell surface binding activities, comparable to mAbs. Triple-negative breast cancer (TNBC) cells and a xenograft model were used to test the potency of BsAbs and other biological activities. Results showed that anti-EGFR/PD-L1 BsAbs exhibited in vitro and in vivo antitumor proliferation activity, but there was a difference in the potencies of the respective BsAb formats (DVD-Ig and KIH) when different cells or assays were used. This study provides evidence that the potency of the BsAbs targeting the same antigens can be affected by the respective molecular features, and selection of appropriate cell lines and assays is critically important for the assay development and potency testing of BsAbs. MDPI 2022-06-29 /pmc/articles/PMC9325241/ /pubmed/35890277 http://dx.doi.org/10.3390/pharmaceutics14071381 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mohan, Nishant
Agrawal, Atul
Shen, Yi
Winarski, Katie L.
Endo, Yukinori
Dokmanovic, Milos
Schmiel, Deborah
Zheng, Jiwen
Rotstein, David S.
Pelosof, Lorraine C.
Wu, Wen Jin
Comparative Characterization of Different Molecular Formats of Bispecific Antibodies Targeting EGFR and PD-L1
title Comparative Characterization of Different Molecular Formats of Bispecific Antibodies Targeting EGFR and PD-L1
title_full Comparative Characterization of Different Molecular Formats of Bispecific Antibodies Targeting EGFR and PD-L1
title_fullStr Comparative Characterization of Different Molecular Formats of Bispecific Antibodies Targeting EGFR and PD-L1
title_full_unstemmed Comparative Characterization of Different Molecular Formats of Bispecific Antibodies Targeting EGFR and PD-L1
title_short Comparative Characterization of Different Molecular Formats of Bispecific Antibodies Targeting EGFR and PD-L1
title_sort comparative characterization of different molecular formats of bispecific antibodies targeting egfr and pd-l1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325241/
https://www.ncbi.nlm.nih.gov/pubmed/35890277
http://dx.doi.org/10.3390/pharmaceutics14071381
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