How to avoid misinterpretation of dual reporter gene assay data affected by cell damage

The activity of nuclear receptors (e.g., pregnane x receptor, PXR) can be assessed by luminescence-based dual reporter gene assays. Under most conditions, receptor-activated firefly luminescence is normalized to Renilla luminescence, which is triggered by a constitutively active promoter. Simultaneo...

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Detalles Bibliográficos
Autores principales: Nilles, Julie, Weiss, Johanna, Haefeli, Walter E., Ruez, Stephanie, Theile, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Molecular Toxicology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325833/
https://www.ncbi.nlm.nih.gov/pubmed/35678845
http://dx.doi.org/10.1007/s00204-022-03323-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325833/
https://www.ncbi.nlm.nih.gov/pubmed/35678845
http://dx.doi.org/10.1007/s00204-022-03323-0

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