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Monitoring the Progression of Cell-Free Expression of Microbial Rhodopsins by Surface Enhanced IR Spectroscopy: Resolving a Branch Point for Successful/Unsuccessful Folding

The translocon-unassisted folding process of transmembrane domains of the microbial rhodopsins sensory rhodopsin I (HsSRI) and II (HsSRII), channelrhodopsin II (CrChR2), and bacteriorhodopsin (HsBR) during cell-free expression has been investigated by Surface-Enhanced Infrared Absorption Spectroscop...

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Detalles Bibliográficos
Autores principales: Ataka, Kenichi, Baumann, Axel, Chen, Jheng-Liang, Redlich, Aoife, Heberle, Joachim, Schlesinger, Ramona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329800/
https://www.ncbi.nlm.nih.gov/pubmed/35911953
http://dx.doi.org/10.3389/fmolb.2022.929285