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Solvent Precipitation SP3 (SP4) Enhances Recovery for Proteomics Sample Preparation without Magnetic Beads

[Image: see text] Complete, reproducible extraction of protein material is essential for comprehensive and unbiased proteome analyses. A current gold standard is single-pot, solid-phase-enhanced sample preparation (SP3), in which organic solvent and magnetic beads are used to denature and capture pr...

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Detalles Bibliográficos
Autores principales: Johnston, Harvey E., Yadav, Kranthikumar, Kirkpatrick, Joanna M., Biggs, George S., Oxley, David, Kramer, Holger B., Samant, Rahul S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330274/
https://www.ncbi.nlm.nih.gov/pubmed/35848328
http://dx.doi.org/10.1021/acs.analchem.1c04200