The efficiency of Nextera XT tagmentation depends on G and C bases in the binding motif leading to uneven coverage in bacterial species with low and neutral GC-content

Whole-genome sequencing (WGS) is becoming the new standard for bacterial high-resolution typing and the performance of laboratories is being evaluated in interlaboratory comparisons. The use of the Illumina Nextera XT library preparation kit has been found to be associated with poorer performance du...

Descripción completa

Detalles Bibliográficos
Autores principales: Segerman, Bo, Ástvaldsson, Ásgeir, Mustafa, Linda, Skarin, Joakim, Skarin, Hanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330563/
https://www.ncbi.nlm.nih.gov/pubmed/35910628
http://dx.doi.org/10.3389/fmicb.2022.944770

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330563/
https://www.ncbi.nlm.nih.gov/pubmed/35910628
http://dx.doi.org/10.3389/fmicb.2022.944770

Ejemplares similares