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Isolated Variable Domains of an Antibody Can Assemble on Blood Coagulation Factor VIII into a Functional Fv-like Complex

Single-chain variable fragments (scFv) are antigen-recognizing variable fragments of antibodies (FV) where both subunits (V(L) and V(H)) are connected via an artificial linker. One particular scFv, iKM33, directed against blood coagulation factor VIII (FVIII) was shown to inhibit major FVIII functio...

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Autores principales: Shestopal, Svetlana A., Parunov, Leonid A., Olivares, Philip, Chun, Haarin, Ovanesov, Mikhail V., Pettersson, John R., Sarafanov, Andrey G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330781/
https://www.ncbi.nlm.nih.gov/pubmed/35897712
http://dx.doi.org/10.3390/ijms23158134
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author Shestopal, Svetlana A.
Parunov, Leonid A.
Olivares, Philip
Chun, Haarin
Ovanesov, Mikhail V.
Pettersson, John R.
Sarafanov, Andrey G.
author_facet Shestopal, Svetlana A.
Parunov, Leonid A.
Olivares, Philip
Chun, Haarin
Ovanesov, Mikhail V.
Pettersson, John R.
Sarafanov, Andrey G.
author_sort Shestopal, Svetlana A.
collection PubMed
description Single-chain variable fragments (scFv) are antigen-recognizing variable fragments of antibodies (FV) where both subunits (V(L) and V(H)) are connected via an artificial linker. One particular scFv, iKM33, directed against blood coagulation factor VIII (FVIII) was shown to inhibit major FVIII functions and is useful in FVIII research. We aimed to investigate the properties of iKM33 enabled with protease-dependent disintegration. Three variants of iKM33 bearing thrombin cleavage sites within the linker were expressed using a baculovirus system and purified by two-step chromatography. All proteins retained strong binding to FVIII by surface plasmon resonance, and upon thrombin cleavage, dissociated into V(L) and V(H) as shown by size-exclusion chromatography. However, in FVIII activity and low-density lipoprotein receptor-related protein 1 binding assays, the thrombin-cleaved iKM33 variants were still inhibitory. In a pull-down assay using an FVIII-affinity sorbent, the isolated V(H), a mixture of V(L) and V(H), and intact iKM33 were carried over via FVIII analyzed by electrophoresis. We concluded that the isolated V(L) and V(H) assembled into scFv-like heterodimer on FVIII, and the isolated V(H) alone also bound FVIII. We discuss the potential use of both protease-cleavable scFvs and isolated Fv subunits retaining high affinity to the antigens in various practical applications such as therapeutics, diagnostics, and research.
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spelling pubmed-93307812022-07-29 Isolated Variable Domains of an Antibody Can Assemble on Blood Coagulation Factor VIII into a Functional Fv-like Complex Shestopal, Svetlana A. Parunov, Leonid A. Olivares, Philip Chun, Haarin Ovanesov, Mikhail V. Pettersson, John R. Sarafanov, Andrey G. Int J Mol Sci Article Single-chain variable fragments (scFv) are antigen-recognizing variable fragments of antibodies (FV) where both subunits (V(L) and V(H)) are connected via an artificial linker. One particular scFv, iKM33, directed against blood coagulation factor VIII (FVIII) was shown to inhibit major FVIII functions and is useful in FVIII research. We aimed to investigate the properties of iKM33 enabled with protease-dependent disintegration. Three variants of iKM33 bearing thrombin cleavage sites within the linker were expressed using a baculovirus system and purified by two-step chromatography. All proteins retained strong binding to FVIII by surface plasmon resonance, and upon thrombin cleavage, dissociated into V(L) and V(H) as shown by size-exclusion chromatography. However, in FVIII activity and low-density lipoprotein receptor-related protein 1 binding assays, the thrombin-cleaved iKM33 variants were still inhibitory. In a pull-down assay using an FVIII-affinity sorbent, the isolated V(H), a mixture of V(L) and V(H), and intact iKM33 were carried over via FVIII analyzed by electrophoresis. We concluded that the isolated V(L) and V(H) assembled into scFv-like heterodimer on FVIII, and the isolated V(H) alone also bound FVIII. We discuss the potential use of both protease-cleavable scFvs and isolated Fv subunits retaining high affinity to the antigens in various practical applications such as therapeutics, diagnostics, and research. MDPI 2022-07-23 /pmc/articles/PMC9330781/ /pubmed/35897712 http://dx.doi.org/10.3390/ijms23158134 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shestopal, Svetlana A.
Parunov, Leonid A.
Olivares, Philip
Chun, Haarin
Ovanesov, Mikhail V.
Pettersson, John R.
Sarafanov, Andrey G.
Isolated Variable Domains of an Antibody Can Assemble on Blood Coagulation Factor VIII into a Functional Fv-like Complex
title Isolated Variable Domains of an Antibody Can Assemble on Blood Coagulation Factor VIII into a Functional Fv-like Complex
title_full Isolated Variable Domains of an Antibody Can Assemble on Blood Coagulation Factor VIII into a Functional Fv-like Complex
title_fullStr Isolated Variable Domains of an Antibody Can Assemble on Blood Coagulation Factor VIII into a Functional Fv-like Complex
title_full_unstemmed Isolated Variable Domains of an Antibody Can Assemble on Blood Coagulation Factor VIII into a Functional Fv-like Complex
title_short Isolated Variable Domains of an Antibody Can Assemble on Blood Coagulation Factor VIII into a Functional Fv-like Complex
title_sort isolated variable domains of an antibody can assemble on blood coagulation factor viii into a functional fv-like complex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330781/
https://www.ncbi.nlm.nih.gov/pubmed/35897712
http://dx.doi.org/10.3390/ijms23158134
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