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Virulent Phages Isolated from a Smear-Ripened Cheese Are Also Detected in Reservoirs of the Cheese Factory

Smear-ripened cheeses host complex microbial communities that play a crucial role in the ripening process. Although bacteriophages have been frequently isolated from dairy products, their diversity and ecological role in such this type of cheese remain underexplored. In order to fill this gap, the m...

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Autores principales: Paillet, Thomas, Lossouarn, Julien, Figueroa, Clarisse, Midoux, Cédric, Rué, Olivier, Petit, Marie-Agnès, Dugat-Bony, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331655/
https://www.ncbi.nlm.nih.gov/pubmed/35893685
http://dx.doi.org/10.3390/v14081620
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author Paillet, Thomas
Lossouarn, Julien
Figueroa, Clarisse
Midoux, Cédric
Rué, Olivier
Petit, Marie-Agnès
Dugat-Bony, Eric
author_facet Paillet, Thomas
Lossouarn, Julien
Figueroa, Clarisse
Midoux, Cédric
Rué, Olivier
Petit, Marie-Agnès
Dugat-Bony, Eric
author_sort Paillet, Thomas
collection PubMed
description Smear-ripened cheeses host complex microbial communities that play a crucial role in the ripening process. Although bacteriophages have been frequently isolated from dairy products, their diversity and ecological role in such this type of cheese remain underexplored. In order to fill this gap, the main objective of this study was to isolate and characterize bacteriophages from the rind of a smear-ripened cheese. Thus, viral particles extracted from the cheese rind were tested through a spot assay against a collection of bacteria isolated from the same cheese and identified by sequencing the full-length small subunit ribosomal RNA gene. In total, five virulent bacteriophages infecting Brevibacterium aurantiacum, Glutamicibacter arilaitensis, Leuconostoc falkenbergense and Psychrobacter aquimaris species were obtained. All exhibit a narrow host range, being only able to infect a few cheese-rind isolates within the same species. The complete genome of each phage was sequenced using both Nanopore and Illumina technologies, assembled and annotated. A sequence comparison with known phages revealed that four of them may represent at least new genera. The distribution of the five virulent phages into the dairy-plant environment was also investigated by PCR, and three potential reservoirs were identified. This work provides new knowledge on the cheese rind viral community and an overview of the distribution of phages within a cheese factory.
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spelling pubmed-93316552022-07-29 Virulent Phages Isolated from a Smear-Ripened Cheese Are Also Detected in Reservoirs of the Cheese Factory Paillet, Thomas Lossouarn, Julien Figueroa, Clarisse Midoux, Cédric Rué, Olivier Petit, Marie-Agnès Dugat-Bony, Eric Viruses Article Smear-ripened cheeses host complex microbial communities that play a crucial role in the ripening process. Although bacteriophages have been frequently isolated from dairy products, their diversity and ecological role in such this type of cheese remain underexplored. In order to fill this gap, the main objective of this study was to isolate and characterize bacteriophages from the rind of a smear-ripened cheese. Thus, viral particles extracted from the cheese rind were tested through a spot assay against a collection of bacteria isolated from the same cheese and identified by sequencing the full-length small subunit ribosomal RNA gene. In total, five virulent bacteriophages infecting Brevibacterium aurantiacum, Glutamicibacter arilaitensis, Leuconostoc falkenbergense and Psychrobacter aquimaris species were obtained. All exhibit a narrow host range, being only able to infect a few cheese-rind isolates within the same species. The complete genome of each phage was sequenced using both Nanopore and Illumina technologies, assembled and annotated. A sequence comparison with known phages revealed that four of them may represent at least new genera. The distribution of the five virulent phages into the dairy-plant environment was also investigated by PCR, and three potential reservoirs were identified. This work provides new knowledge on the cheese rind viral community and an overview of the distribution of phages within a cheese factory. MDPI 2022-07-25 /pmc/articles/PMC9331655/ /pubmed/35893685 http://dx.doi.org/10.3390/v14081620 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Paillet, Thomas
Lossouarn, Julien
Figueroa, Clarisse
Midoux, Cédric
Rué, Olivier
Petit, Marie-Agnès
Dugat-Bony, Eric
Virulent Phages Isolated from a Smear-Ripened Cheese Are Also Detected in Reservoirs of the Cheese Factory
title Virulent Phages Isolated from a Smear-Ripened Cheese Are Also Detected in Reservoirs of the Cheese Factory
title_full Virulent Phages Isolated from a Smear-Ripened Cheese Are Also Detected in Reservoirs of the Cheese Factory
title_fullStr Virulent Phages Isolated from a Smear-Ripened Cheese Are Also Detected in Reservoirs of the Cheese Factory
title_full_unstemmed Virulent Phages Isolated from a Smear-Ripened Cheese Are Also Detected in Reservoirs of the Cheese Factory
title_short Virulent Phages Isolated from a Smear-Ripened Cheese Are Also Detected in Reservoirs of the Cheese Factory
title_sort virulent phages isolated from a smear-ripened cheese are also detected in reservoirs of the cheese factory
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9331655/
https://www.ncbi.nlm.nih.gov/pubmed/35893685
http://dx.doi.org/10.3390/v14081620
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