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Neutrophil Elastase Increases Vascular Permeability and Leukocyte Transmigration in Cultured Endothelial Cells and Obese Mice

Neutrophil elastase (NE) plays a pivotal role in inflammation. However, the mechanism underlying NE-mediated inflammation in obesity remains unclear. Here, we report that NE activates protease-activated receptor-2 (PAR2), stimulates actin filament (F-actin) formation, decreases intercellular junctio...

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Autores principales: Ushakumari, Chinchu Jagadan, Zhou, Qiong L., Wang, Yu-Hua, Na, Sijia, Rigor, Michael C., Zhou, Cindy Y., Kroll, Max K., Lin, Benjamin D., Jiang, Zhen Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332277/
https://www.ncbi.nlm.nih.gov/pubmed/35892585
http://dx.doi.org/10.3390/cells11152288
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author Ushakumari, Chinchu Jagadan
Zhou, Qiong L.
Wang, Yu-Hua
Na, Sijia
Rigor, Michael C.
Zhou, Cindy Y.
Kroll, Max K.
Lin, Benjamin D.
Jiang, Zhen Y.
author_facet Ushakumari, Chinchu Jagadan
Zhou, Qiong L.
Wang, Yu-Hua
Na, Sijia
Rigor, Michael C.
Zhou, Cindy Y.
Kroll, Max K.
Lin, Benjamin D.
Jiang, Zhen Y.
author_sort Ushakumari, Chinchu Jagadan
collection PubMed
description Neutrophil elastase (NE) plays a pivotal role in inflammation. However, the mechanism underlying NE-mediated inflammation in obesity remains unclear. Here, we report that NE activates protease-activated receptor-2 (PAR2), stimulates actin filament (F-actin) formation, decreases intercellular junction molecule VE-cadherin expression, and increases the permeability of human arterial endothelial cells (hECs). NE also prompts degradation of VE-cadherin and its binding proteins p120- and β-catenins via MG132-sensitive proteasomes. NE stimulates phosphorylation of myosin light-chain (MLC) and its regulator myosin phosphatase target subunit-1 (MYPT1), a target of Rho kinase (ROCK). Inhibitors of PAR2 and ROCK prohibit NE-induced F-actin formation, MLC phosphorylation, and VE-cadherin reduction in hECs, and impede monocyte transmigration through hEC monolayer pretreated with either neutrophils or NE. Further, administration of an NE inhibitor GW311616A significantly attenuates vascular leakage, leukocyte infiltration, and the expression of proinflammatory cytokines in the white adipose tissue from high-fat diet (HFD)-induced obese mice. Likewise, NE-deficient mice are resistant to HFD-induced vascular leakage in the heart. Together, NE regulates actomyosin cytoskeleton activity and VE-cadherin expression by activating PAR2 signaling in the endothelial cells, leading to increased vascular permeability and leukocyte extravasation. Hence, inhibition of NE is a potential approach to mitigate vascular injury and leukocyte infiltration in obesity-related systemic inflammation.
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spelling pubmed-93322772022-07-29 Neutrophil Elastase Increases Vascular Permeability and Leukocyte Transmigration in Cultured Endothelial Cells and Obese Mice Ushakumari, Chinchu Jagadan Zhou, Qiong L. Wang, Yu-Hua Na, Sijia Rigor, Michael C. Zhou, Cindy Y. Kroll, Max K. Lin, Benjamin D. Jiang, Zhen Y. Cells Article Neutrophil elastase (NE) plays a pivotal role in inflammation. However, the mechanism underlying NE-mediated inflammation in obesity remains unclear. Here, we report that NE activates protease-activated receptor-2 (PAR2), stimulates actin filament (F-actin) formation, decreases intercellular junction molecule VE-cadherin expression, and increases the permeability of human arterial endothelial cells (hECs). NE also prompts degradation of VE-cadherin and its binding proteins p120- and β-catenins via MG132-sensitive proteasomes. NE stimulates phosphorylation of myosin light-chain (MLC) and its regulator myosin phosphatase target subunit-1 (MYPT1), a target of Rho kinase (ROCK). Inhibitors of PAR2 and ROCK prohibit NE-induced F-actin formation, MLC phosphorylation, and VE-cadherin reduction in hECs, and impede monocyte transmigration through hEC monolayer pretreated with either neutrophils or NE. Further, administration of an NE inhibitor GW311616A significantly attenuates vascular leakage, leukocyte infiltration, and the expression of proinflammatory cytokines in the white adipose tissue from high-fat diet (HFD)-induced obese mice. Likewise, NE-deficient mice are resistant to HFD-induced vascular leakage in the heart. Together, NE regulates actomyosin cytoskeleton activity and VE-cadherin expression by activating PAR2 signaling in the endothelial cells, leading to increased vascular permeability and leukocyte extravasation. Hence, inhibition of NE is a potential approach to mitigate vascular injury and leukocyte infiltration in obesity-related systemic inflammation. MDPI 2022-07-25 /pmc/articles/PMC9332277/ /pubmed/35892585 http://dx.doi.org/10.3390/cells11152288 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ushakumari, Chinchu Jagadan
Zhou, Qiong L.
Wang, Yu-Hua
Na, Sijia
Rigor, Michael C.
Zhou, Cindy Y.
Kroll, Max K.
Lin, Benjamin D.
Jiang, Zhen Y.
Neutrophil Elastase Increases Vascular Permeability and Leukocyte Transmigration in Cultured Endothelial Cells and Obese Mice
title Neutrophil Elastase Increases Vascular Permeability and Leukocyte Transmigration in Cultured Endothelial Cells and Obese Mice
title_full Neutrophil Elastase Increases Vascular Permeability and Leukocyte Transmigration in Cultured Endothelial Cells and Obese Mice
title_fullStr Neutrophil Elastase Increases Vascular Permeability and Leukocyte Transmigration in Cultured Endothelial Cells and Obese Mice
title_full_unstemmed Neutrophil Elastase Increases Vascular Permeability and Leukocyte Transmigration in Cultured Endothelial Cells and Obese Mice
title_short Neutrophil Elastase Increases Vascular Permeability and Leukocyte Transmigration in Cultured Endothelial Cells and Obese Mice
title_sort neutrophil elastase increases vascular permeability and leukocyte transmigration in cultured endothelial cells and obese mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332277/
https://www.ncbi.nlm.nih.gov/pubmed/35892585
http://dx.doi.org/10.3390/cells11152288
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