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Quantitation of MicroRNA-155 in Human Cells by Heterogeneous Enzyme-Linked Oligonucleotide Assay Coupled with Mismatched Catalytic Hairpin Assembly Reaction

In the present work, we describe the development of a chemiluminescent enzyme-linked oligonucleotide assay coupled with mismatched catalytic hairpin assembly (mCHA) amplification for the quantitative determination of microRNA-155. To improve its sensitivity, a polymerase-free mCHA reaction was appli...

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Detalles Bibliográficos
Autores principales: Bodulev, Oleg L., Galkin, Ivan I., Zhao, Shulin, Pletyushkina, Olga Y., Sakharov, Ivan Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332365/
https://www.ncbi.nlm.nih.gov/pubmed/35892467
http://dx.doi.org/10.3390/bios12080570