Duplex Labeling and Manipulation of Neuronal Proteins Using Sequential CRISPR/Cas9 Gene Editing

CRISPR/Cas9-mediated knock-in methods enable the labeling of individual endogenous proteins to faithfully determine their spatiotemporal distribution in cells. However, reliable multiplexing of knock-in events in neurons remains challenging because of cross talk between editing events. To overcome t...

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Detalles Bibliográficos
Autores principales: Droogers, Wouter J., Willems, Jelmer, MacGillavry, Harold D., de Jong, Arthur P. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for Neuroscience 2022
Materias:
Research Article: Methods/New Tools
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9333357/
https://www.ncbi.nlm.nih.gov/pubmed/35851300
http://dx.doi.org/10.1523/ENEURO.0056-22.2022

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9333357/
https://www.ncbi.nlm.nih.gov/pubmed/35851300
http://dx.doi.org/10.1523/ENEURO.0056-22.2022

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