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De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we...

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Autores principales: Tetreau, Guillaume, Sawaya, Michael R., De Zitter, Elke, Andreeva, Elena A., Banneville, Anne-Sophie, Schibrowsky, Natalie A., Coquelle, Nicolas, Brewster, Aaron S., Grünbein, Marie Luise, Kovacs, Gabriela Nass, Hunter, Mark S., Kloos, Marco, Sierra, Raymond G., Schiro, Giorgio, Qiao, Pei, Stricker, Myriam, Bideshi, Dennis, Young, Iris D., Zala, Ninon, Engilberge, Sylvain, Gorel, Alexander, Signor, Luca, Teulon, Jean-Marie, Hilpert, Mario, Foucar, Lutz, Bielecki, Johan, Bean, Richard, de Wijn, Raphael, Sato, Tokushi, Kirkwood, Henry, Letrun, Romain, Batyuk, Alexander, Snigireva, Irina, Fenel, Daphna, Schubert, Robin, Canfield, Ethan J., Alba, Mario M., Laporte, Frédéric, Després, Laurence, Bacia, Maria, Roux, Amandine, Chapelle, Christian, Riobé, François, Maury, Olivier, Ling, Wai Li, Boutet, Sébastien, Mancuso, Adrian, Gutsche, Irina, Girard, Eric, Barends, Thomas R. M., Pellequer, Jean-Luc, Park, Hyun-Woo, Laganowsky, Arthur D., Rodriguez, Jose, Burghammer, Manfred, Shoeman, Robert L., Doak, R. Bruce, Weik, Martin, Sauter, Nicholas K., Federici, Brian, Cascio, Duilio, Schlichting, Ilme, Colletier, Jacques-Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334358/
https://www.ncbi.nlm.nih.gov/pubmed/35902572
http://dx.doi.org/10.1038/s41467-022-31746-x
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author Tetreau, Guillaume
Sawaya, Michael R.
De Zitter, Elke
Andreeva, Elena A.
Banneville, Anne-Sophie
Schibrowsky, Natalie A.
Coquelle, Nicolas
Brewster, Aaron S.
Grünbein, Marie Luise
Kovacs, Gabriela Nass
Hunter, Mark S.
Kloos, Marco
Sierra, Raymond G.
Schiro, Giorgio
Qiao, Pei
Stricker, Myriam
Bideshi, Dennis
Young, Iris D.
Zala, Ninon
Engilberge, Sylvain
Gorel, Alexander
Signor, Luca
Teulon, Jean-Marie
Hilpert, Mario
Foucar, Lutz
Bielecki, Johan
Bean, Richard
de Wijn, Raphael
Sato, Tokushi
Kirkwood, Henry
Letrun, Romain
Batyuk, Alexander
Snigireva, Irina
Fenel, Daphna
Schubert, Robin
Canfield, Ethan J.
Alba, Mario M.
Laporte, Frédéric
Després, Laurence
Bacia, Maria
Roux, Amandine
Chapelle, Christian
Riobé, François
Maury, Olivier
Ling, Wai Li
Boutet, Sébastien
Mancuso, Adrian
Gutsche, Irina
Girard, Eric
Barends, Thomas R. M.
Pellequer, Jean-Luc
Park, Hyun-Woo
Laganowsky, Arthur D.
Rodriguez, Jose
Burghammer, Manfred
Shoeman, Robert L.
Doak, R. Bruce
Weik, Martin
Sauter, Nicholas K.
Federici, Brian
Cascio, Duilio
Schlichting, Ilme
Colletier, Jacques-Philippe
author_facet Tetreau, Guillaume
Sawaya, Michael R.
De Zitter, Elke
Andreeva, Elena A.
Banneville, Anne-Sophie
Schibrowsky, Natalie A.
Coquelle, Nicolas
Brewster, Aaron S.
Grünbein, Marie Luise
Kovacs, Gabriela Nass
Hunter, Mark S.
Kloos, Marco
Sierra, Raymond G.
Schiro, Giorgio
Qiao, Pei
Stricker, Myriam
Bideshi, Dennis
Young, Iris D.
Zala, Ninon
Engilberge, Sylvain
Gorel, Alexander
Signor, Luca
Teulon, Jean-Marie
Hilpert, Mario
Foucar, Lutz
Bielecki, Johan
Bean, Richard
de Wijn, Raphael
Sato, Tokushi
Kirkwood, Henry
Letrun, Romain
Batyuk, Alexander
Snigireva, Irina
Fenel, Daphna
Schubert, Robin
Canfield, Ethan J.
Alba, Mario M.
Laporte, Frédéric
Després, Laurence
Bacia, Maria
Roux, Amandine
Chapelle, Christian
Riobé, François
Maury, Olivier
Ling, Wai Li
Boutet, Sébastien
Mancuso, Adrian
Gutsche, Irina
Girard, Eric
Barends, Thomas R. M.
Pellequer, Jean-Luc
Park, Hyun-Woo
Laganowsky, Arthur D.
Rodriguez, Jose
Burghammer, Manfred
Shoeman, Robert L.
Doak, R. Bruce
Weik, Martin
Sauter, Nicholas K.
Federici, Brian
Cascio, Duilio
Schlichting, Ilme
Colletier, Jacques-Philippe
author_sort Tetreau, Guillaume
collection PubMed
description Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.
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spelling pubmed-93343582022-07-30 De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals Tetreau, Guillaume Sawaya, Michael R. De Zitter, Elke Andreeva, Elena A. Banneville, Anne-Sophie Schibrowsky, Natalie A. Coquelle, Nicolas Brewster, Aaron S. Grünbein, Marie Luise Kovacs, Gabriela Nass Hunter, Mark S. Kloos, Marco Sierra, Raymond G. Schiro, Giorgio Qiao, Pei Stricker, Myriam Bideshi, Dennis Young, Iris D. Zala, Ninon Engilberge, Sylvain Gorel, Alexander Signor, Luca Teulon, Jean-Marie Hilpert, Mario Foucar, Lutz Bielecki, Johan Bean, Richard de Wijn, Raphael Sato, Tokushi Kirkwood, Henry Letrun, Romain Batyuk, Alexander Snigireva, Irina Fenel, Daphna Schubert, Robin Canfield, Ethan J. Alba, Mario M. Laporte, Frédéric Després, Laurence Bacia, Maria Roux, Amandine Chapelle, Christian Riobé, François Maury, Olivier Ling, Wai Li Boutet, Sébastien Mancuso, Adrian Gutsche, Irina Girard, Eric Barends, Thomas R. M. Pellequer, Jean-Luc Park, Hyun-Woo Laganowsky, Arthur D. Rodriguez, Jose Burghammer, Manfred Shoeman, Robert L. Doak, R. Bruce Weik, Martin Sauter, Nicholas K. Federici, Brian Cascio, Duilio Schlichting, Ilme Colletier, Jacques-Philippe Nat Commun Article Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control. Nature Publishing Group UK 2022-07-28 /pmc/articles/PMC9334358/ /pubmed/35902572 http://dx.doi.org/10.1038/s41467-022-31746-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Tetreau, Guillaume
Sawaya, Michael R.
De Zitter, Elke
Andreeva, Elena A.
Banneville, Anne-Sophie
Schibrowsky, Natalie A.
Coquelle, Nicolas
Brewster, Aaron S.
Grünbein, Marie Luise
Kovacs, Gabriela Nass
Hunter, Mark S.
Kloos, Marco
Sierra, Raymond G.
Schiro, Giorgio
Qiao, Pei
Stricker, Myriam
Bideshi, Dennis
Young, Iris D.
Zala, Ninon
Engilberge, Sylvain
Gorel, Alexander
Signor, Luca
Teulon, Jean-Marie
Hilpert, Mario
Foucar, Lutz
Bielecki, Johan
Bean, Richard
de Wijn, Raphael
Sato, Tokushi
Kirkwood, Henry
Letrun, Romain
Batyuk, Alexander
Snigireva, Irina
Fenel, Daphna
Schubert, Robin
Canfield, Ethan J.
Alba, Mario M.
Laporte, Frédéric
Després, Laurence
Bacia, Maria
Roux, Amandine
Chapelle, Christian
Riobé, François
Maury, Olivier
Ling, Wai Li
Boutet, Sébastien
Mancuso, Adrian
Gutsche, Irina
Girard, Eric
Barends, Thomas R. M.
Pellequer, Jean-Luc
Park, Hyun-Woo
Laganowsky, Arthur D.
Rodriguez, Jose
Burghammer, Manfred
Shoeman, Robert L.
Doak, R. Bruce
Weik, Martin
Sauter, Nicholas K.
Federici, Brian
Cascio, Duilio
Schlichting, Ilme
Colletier, Jacques-Philippe
De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals
title De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals
title_full De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals
title_fullStr De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals
title_full_unstemmed De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals
title_short De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals
title_sort de novo determination of mosquitocidal cry11aa and cry11ba structures from naturally-occurring nanocrystals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334358/
https://www.ncbi.nlm.nih.gov/pubmed/35902572
http://dx.doi.org/10.1038/s41467-022-31746-x
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