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Propidium Monoazide Combined With RT-qPCR Detects Infectivity of Porcine Epidemic Diarrhea Virus

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) allows sensitive detection of viral particles and viruses in epidemic samples but it cannot discriminate noninfectious viruses from infectious ones. Propidium monoazide (PMA) coupled with quantitative polymerase chain reaction (q...

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Detalles Bibliográficos
Autores principales: Liang, Gong, Long, Yunzhi, Li, Qianqian, Yang, Liu, Huang, Ying, Yu, Daobing, Song, Wenbo, Zhou, Mingguang, Xu, Gaoyuan, Huang, Chao, Tang, Xibiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334817/
https://www.ncbi.nlm.nih.gov/pubmed/35909686
http://dx.doi.org/10.3389/fvets.2022.931392