Toward a Topology-Based Therapeutic Design of Membrane Proteins: Validation of NaPi2b Topology in Live Ovarian Cancer Cells

NaPi2b is a sodium-dependent phosphate transporter that belongs to the SLC34 family of transporters which is mainly responsible for phosphate homeostasis in humans. Although NaPi2b is widely expressed in normal tissues, its overexpression has been demonstrated in ovarian, lung, and other cancers. A...

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Autores principales: Bulatova, Leisan, Savenkova, Daria, Nurgalieva, Alsina, Reshetnikova, Daria, Timonina, Arina, Skripova, Vera, Bogdanov, Mikhail, Kiyamova, Ramziya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335355/
https://www.ncbi.nlm.nih.gov/pubmed/35911971
http://dx.doi.org/10.3389/fmolb.2022.895911
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author Bulatova, Leisan
Savenkova, Daria
Nurgalieva, Alsina
Reshetnikova, Daria
Timonina, Arina
Skripova, Vera
Bogdanov, Mikhail
Kiyamova, Ramziya
author_facet Bulatova, Leisan
Savenkova, Daria
Nurgalieva, Alsina
Reshetnikova, Daria
Timonina, Arina
Skripova, Vera
Bogdanov, Mikhail
Kiyamova, Ramziya
author_sort Bulatova, Leisan
collection PubMed
description NaPi2b is a sodium-dependent phosphate transporter that belongs to the SLC34 family of transporters which is mainly responsible for phosphate homeostasis in humans. Although NaPi2b is widely expressed in normal tissues, its overexpression has been demonstrated in ovarian, lung, and other cancers. A valuable set of antibodies, including L2 (20/3) and MX35, and its humanized versions react strongly with an antigen on the surface of ovarian and other carcinoma cells. Although the topology of NaPi2b was predicted in silico, no direct experimental data are available for the orientation of NaPi2b extracellular domains in cancer cells. The presented results of antibody mapping of untagged NaPi2b in live ovarian carcinoma cells OVCAR-4 provide a platform for current and future epitope-based cancer therapies and serological diagnostics.
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spelling pubmed-93353552022-07-30 Toward a Topology-Based Therapeutic Design of Membrane Proteins: Validation of NaPi2b Topology in Live Ovarian Cancer Cells Bulatova, Leisan Savenkova, Daria Nurgalieva, Alsina Reshetnikova, Daria Timonina, Arina Skripova, Vera Bogdanov, Mikhail Kiyamova, Ramziya Front Mol Biosci Molecular Biosciences NaPi2b is a sodium-dependent phosphate transporter that belongs to the SLC34 family of transporters which is mainly responsible for phosphate homeostasis in humans. Although NaPi2b is widely expressed in normal tissues, its overexpression has been demonstrated in ovarian, lung, and other cancers. A valuable set of antibodies, including L2 (20/3) and MX35, and its humanized versions react strongly with an antigen on the surface of ovarian and other carcinoma cells. Although the topology of NaPi2b was predicted in silico, no direct experimental data are available for the orientation of NaPi2b extracellular domains in cancer cells. The presented results of antibody mapping of untagged NaPi2b in live ovarian carcinoma cells OVCAR-4 provide a platform for current and future epitope-based cancer therapies and serological diagnostics. Frontiers Media S.A. 2022-07-15 /pmc/articles/PMC9335355/ /pubmed/35911971 http://dx.doi.org/10.3389/fmolb.2022.895911 Text en Copyright © 2022 Bulatova, Savenkova, Nurgalieva, Reshetnikova, Timonina, Skripova, Bogdanov and Kiyamova. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Bulatova, Leisan
Savenkova, Daria
Nurgalieva, Alsina
Reshetnikova, Daria
Timonina, Arina
Skripova, Vera
Bogdanov, Mikhail
Kiyamova, Ramziya
Toward a Topology-Based Therapeutic Design of Membrane Proteins: Validation of NaPi2b Topology in Live Ovarian Cancer Cells
title Toward a Topology-Based Therapeutic Design of Membrane Proteins: Validation of NaPi2b Topology in Live Ovarian Cancer Cells
title_full Toward a Topology-Based Therapeutic Design of Membrane Proteins: Validation of NaPi2b Topology in Live Ovarian Cancer Cells
title_fullStr Toward a Topology-Based Therapeutic Design of Membrane Proteins: Validation of NaPi2b Topology in Live Ovarian Cancer Cells
title_full_unstemmed Toward a Topology-Based Therapeutic Design of Membrane Proteins: Validation of NaPi2b Topology in Live Ovarian Cancer Cells
title_short Toward a Topology-Based Therapeutic Design of Membrane Proteins: Validation of NaPi2b Topology in Live Ovarian Cancer Cells
title_sort toward a topology-based therapeutic design of membrane proteins: validation of napi2b topology in live ovarian cancer cells
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335355/
https://www.ncbi.nlm.nih.gov/pubmed/35911971
http://dx.doi.org/10.3389/fmolb.2022.895911
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