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New insights into sperm rheotaxis, agglutination and bundle formation in Sharkasi chickens based on an in vitro study
Fertility in birds is dependent on their ability to store adequate populations of viable sperm for extended durations in sperm storage tubules (SSTs). The exact mechanisms by which sperm enter, reside, and egress from the SSTs are still controversial. Sharkasi chicken sperm showed a high tendency to...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338266/ https://www.ncbi.nlm.nih.gov/pubmed/35906270 http://dx.doi.org/10.1038/s41598-022-17037-x |
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author | El-Sherry, Taymour M. Abd-Elhafeez, Hanan H. Sayed, M. A. M. |
author_facet | El-Sherry, Taymour M. Abd-Elhafeez, Hanan H. Sayed, M. A. M. |
author_sort | El-Sherry, Taymour M. |
collection | PubMed |
description | Fertility in birds is dependent on their ability to store adequate populations of viable sperm for extended durations in sperm storage tubules (SSTs). The exact mechanisms by which sperm enter, reside, and egress from the SSTs are still controversial. Sharkasi chicken sperm showed a high tendency to agglutinate, forming motile thread-like bundles comprising many cells. Since it is difficult to observe sperm motility and behavior inside the opaque oviduct, we employed a microfluidic device with a microchannel cross-section resembling close to that of sperm glands allowing for the study of sperm agglutination and motility behavior. This study discusses how sperm bundles are formed, how they move, and what role they may have in extending sperm residency inside the SSTs. We investigated sperm velocity and rheotaxis behavior when a fluid flow was generated inside a microfluidic channel by hydrostatic pressure (flow velocity = 33 µm/s). Spermatozoa tended to swim against the flow (positive rheotaxis) and sperm bundles had significantly lower velocity compared to lonesome sperm. Sperm bundles were observed to swim in a spiral-like motion and to grow in length and thickness as more lonesome sperm are recruited. Sperm bundles were observed approaching and adhering to the sidewalls of the microfluidic channels to avoid being swept with fluid flow velocity > 33 µm/s. Scanning and transmission electron microscopy revealed that sperm bundles were supported by a copious dense substance. The findings show the distinct motility of Sharkasi chicken sperm, as well as sperm's capacity to agglutinate and form motile bundles, which provides a better understanding of long-term sperm storage in the SSTs. |
format | Online Article Text |
id | pubmed-9338266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-93382662022-07-31 New insights into sperm rheotaxis, agglutination and bundle formation in Sharkasi chickens based on an in vitro study El-Sherry, Taymour M. Abd-Elhafeez, Hanan H. Sayed, M. A. M. Sci Rep Article Fertility in birds is dependent on their ability to store adequate populations of viable sperm for extended durations in sperm storage tubules (SSTs). The exact mechanisms by which sperm enter, reside, and egress from the SSTs are still controversial. Sharkasi chicken sperm showed a high tendency to agglutinate, forming motile thread-like bundles comprising many cells. Since it is difficult to observe sperm motility and behavior inside the opaque oviduct, we employed a microfluidic device with a microchannel cross-section resembling close to that of sperm glands allowing for the study of sperm agglutination and motility behavior. This study discusses how sperm bundles are formed, how they move, and what role they may have in extending sperm residency inside the SSTs. We investigated sperm velocity and rheotaxis behavior when a fluid flow was generated inside a microfluidic channel by hydrostatic pressure (flow velocity = 33 µm/s). Spermatozoa tended to swim against the flow (positive rheotaxis) and sperm bundles had significantly lower velocity compared to lonesome sperm. Sperm bundles were observed to swim in a spiral-like motion and to grow in length and thickness as more lonesome sperm are recruited. Sperm bundles were observed approaching and adhering to the sidewalls of the microfluidic channels to avoid being swept with fluid flow velocity > 33 µm/s. Scanning and transmission electron microscopy revealed that sperm bundles were supported by a copious dense substance. The findings show the distinct motility of Sharkasi chicken sperm, as well as sperm's capacity to agglutinate and form motile bundles, which provides a better understanding of long-term sperm storage in the SSTs. Nature Publishing Group UK 2022-07-29 /pmc/articles/PMC9338266/ /pubmed/35906270 http://dx.doi.org/10.1038/s41598-022-17037-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article El-Sherry, Taymour M. Abd-Elhafeez, Hanan H. Sayed, M. A. M. New insights into sperm rheotaxis, agglutination and bundle formation in Sharkasi chickens based on an in vitro study |
title | New insights into sperm rheotaxis, agglutination and bundle formation in Sharkasi chickens based on an in vitro study |
title_full | New insights into sperm rheotaxis, agglutination and bundle formation in Sharkasi chickens based on an in vitro study |
title_fullStr | New insights into sperm rheotaxis, agglutination and bundle formation in Sharkasi chickens based on an in vitro study |
title_full_unstemmed | New insights into sperm rheotaxis, agglutination and bundle formation in Sharkasi chickens based on an in vitro study |
title_short | New insights into sperm rheotaxis, agglutination and bundle formation in Sharkasi chickens based on an in vitro study |
title_sort | new insights into sperm rheotaxis, agglutination and bundle formation in sharkasi chickens based on an in vitro study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338266/ https://www.ncbi.nlm.nih.gov/pubmed/35906270 http://dx.doi.org/10.1038/s41598-022-17037-x |
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