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Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants

Reverse transcription quantitative PCR (RT-qPCR) assays are gold standard in diagnosing SARS-CoV-2 infection and play a major role in viral subtyping for rapid detection and monitoring of important mutations, containing the spread of new virus variants. We wanted to compare RT-qPCR melting curve ana...

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Detalles Bibliográficos
Autores principales: Juul, Sebastian, Spiegelhauer, Malene Roed, Petersen, Mette Neve, Flugt, Katharina Kirkegaard, Hansen, Nikolaj Vestergaard, Larsen, Helene, Jensen, Per Bo, Christensen, Ulf Bech, Petersen, Rasmus Koefoed, Friis-Hansen, Lennart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338320/
https://www.ncbi.nlm.nih.gov/pubmed/35906388
http://dx.doi.org/10.1038/s41598-022-17339-0