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Purification and identification of anti-inflammatory peptides derived from simulated gastrointestinal digests of velvet antler protein (Cervus elaphus Linnaeus)

The objective of this study was to identify anti-inflammatory peptides from simulated gastrointestinal digest (pepsin–pancreatin hydrolysate) of velvet antler protein. The hydrolysate was purified using ultrafiltration and consecutive chromatographic methods. The anti-inflammatory activity of the pu...

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Autores principales: Zhao, Lei, Wang, Xuan, Zhang, Xiao-Lei, Xie, Qiao-Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339547/
https://www.ncbi.nlm.nih.gov/pubmed/28911592
http://dx.doi.org/10.1016/j.jfda.2015.10.003
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author Zhao, Lei
Wang, Xuan
Zhang, Xiao-Lei
Xie, Qiao-Fei
author_facet Zhao, Lei
Wang, Xuan
Zhang, Xiao-Lei
Xie, Qiao-Fei
author_sort Zhao, Lei
collection PubMed
description The objective of this study was to identify anti-inflammatory peptides from simulated gastrointestinal digest (pepsin–pancreatin hydrolysate) of velvet antler protein. The hydrolysate was purified using ultrafiltration and consecutive chromatographic methods. The anti-inflammatory activity of the purified fraction was evaluated by the inhibition of NO production in lipopolysaccharide-induced RAW 264.7 macrophages. Four anti-inflammatory peptides, VH (Val–His), LAN (Leu–Ala–Asn), AL (Ala–Leu), and IA (Ile–Ala), were identified by liquid chromatography/tandem mass spectrometry. Each of these peptides demonstrated a U-shaped dose–effect relationship. VH, LAN, AL and IA showed the strongest anti-inflammatory activities at 200 μg/mL, that is, 15.5%, 13.0%, 16.0% and 11.2% inhibition of lipopolysaccharide-induced NO production, respectively. Additionally, the enhanced NO inhibitory activity was observed for the peptides mixture, indicating the possible synergistic effects. These results suggested that the peptides derived from velvet antler protein could potentially be used as a promising ingredient in functional foods or nutraceuticals against inflammatory diseases.
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spelling pubmed-93395472022-08-09 Purification and identification of anti-inflammatory peptides derived from simulated gastrointestinal digests of velvet antler protein (Cervus elaphus Linnaeus) Zhao, Lei Wang, Xuan Zhang, Xiao-Lei Xie, Qiao-Fei J Food Drug Anal Original Article The objective of this study was to identify anti-inflammatory peptides from simulated gastrointestinal digest (pepsin–pancreatin hydrolysate) of velvet antler protein. The hydrolysate was purified using ultrafiltration and consecutive chromatographic methods. The anti-inflammatory activity of the purified fraction was evaluated by the inhibition of NO production in lipopolysaccharide-induced RAW 264.7 macrophages. Four anti-inflammatory peptides, VH (Val–His), LAN (Leu–Ala–Asn), AL (Ala–Leu), and IA (Ile–Ala), were identified by liquid chromatography/tandem mass spectrometry. Each of these peptides demonstrated a U-shaped dose–effect relationship. VH, LAN, AL and IA showed the strongest anti-inflammatory activities at 200 μg/mL, that is, 15.5%, 13.0%, 16.0% and 11.2% inhibition of lipopolysaccharide-induced NO production, respectively. Additionally, the enhanced NO inhibitory activity was observed for the peptides mixture, indicating the possible synergistic effects. These results suggested that the peptides derived from velvet antler protein could potentially be used as a promising ingredient in functional foods or nutraceuticals against inflammatory diseases. Taiwan Food and Drug Administration 2016-01-05 /pmc/articles/PMC9339547/ /pubmed/28911592 http://dx.doi.org/10.1016/j.jfda.2015.10.003 Text en © 2016 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Zhao, Lei
Wang, Xuan
Zhang, Xiao-Lei
Xie, Qiao-Fei
Purification and identification of anti-inflammatory peptides derived from simulated gastrointestinal digests of velvet antler protein (Cervus elaphus Linnaeus)
title Purification and identification of anti-inflammatory peptides derived from simulated gastrointestinal digests of velvet antler protein (Cervus elaphus Linnaeus)
title_full Purification and identification of anti-inflammatory peptides derived from simulated gastrointestinal digests of velvet antler protein (Cervus elaphus Linnaeus)
title_fullStr Purification and identification of anti-inflammatory peptides derived from simulated gastrointestinal digests of velvet antler protein (Cervus elaphus Linnaeus)
title_full_unstemmed Purification and identification of anti-inflammatory peptides derived from simulated gastrointestinal digests of velvet antler protein (Cervus elaphus Linnaeus)
title_short Purification and identification of anti-inflammatory peptides derived from simulated gastrointestinal digests of velvet antler protein (Cervus elaphus Linnaeus)
title_sort purification and identification of anti-inflammatory peptides derived from simulated gastrointestinal digests of velvet antler protein (cervus elaphus linnaeus)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339547/
https://www.ncbi.nlm.nih.gov/pubmed/28911592
http://dx.doi.org/10.1016/j.jfda.2015.10.003
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