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Improved chemical fixation of lipid-secreting plant cells for transmission electron microscopy

Cultured Lithospermum erythrorhizon cells were fixed with a new fixation method to visualize the metabolism of shikonin derivatives, the lipophilic naphthoquinone pigments in Boraginaceae. The new fixation method combined glutaraldehyde containing malachite green, imidazole–osmium and p-phenylenedia...

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Detalles Bibliográficos
Autores principales: Kiyoto, Shingo, Ichino, Takuji, Awano, Tatsuya, Yazaki, Kazufumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340797/
https://www.ncbi.nlm.nih.gov/pubmed/35388424
http://dx.doi.org/10.1093/jmicro/dfac018