Target residence of Cas9-sgRNA influences DNA double-strand break repair pathway choices in CRISPR/Cas9 genome editing

BACKGROUND: Due to post-cleavage residence of the Cas9-sgRNA complex at its target, Cas9-induced DNA double-strand breaks (DSBs) have to be exposed to engage DSB repair pathways. Target interaction of Cas9-sgRNA determines its target binding affinity and modulates its post-cleavage target residence...

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Detalles Bibliográficos
Autores principales: Liu, Si-Cheng, Feng, Yi-Li, Sun, Xiu-Na, Chen, Ruo-Dan, Liu, Qian, Xiao, Jing-Jing, Zhang, Jin-Na, Huang, Zhi-Cheng, Xiang, Ji-Feng, Chen, Guo-Qiao, Yang, Yi, Lou, Chao, Li, Hao-Dan, Cai, Zhen, Xu, Shi-Ming, Lin, Hui, Xie, An-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341079/
https://www.ncbi.nlm.nih.gov/pubmed/35915475
http://dx.doi.org/10.1186/s13059-022-02736-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341079/
https://www.ncbi.nlm.nih.gov/pubmed/35915475
http://dx.doi.org/10.1186/s13059-022-02736-5

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