Development of PCR-RFLP Technique for Identify Several Members of Fusarium incarnatum-equiseti Species Complex and Fusarium fujikuroi Species Complex

Fusarium incarnatum-equiseti species complex (FIESC) contain over 40 members. The primer pair Smibo1FM/Semi1RM on the RPB2 partial gene has been reported to be able to identify Fusarium semitectum. The F. fujikuroi species complex (FFSC) contains more than 50 members. The F. verticillioides as a mem...

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Autores principales: Pramunadipta, Syafiqa, Widiastuti, Ani, Wibowo, Arif, Suga, Haruhisa, Priyatmojo, Achmadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343909/
https://www.ncbi.nlm.nih.gov/pubmed/35678059
http://dx.doi.org/10.5423/PPJ.NT.12.2021.0184
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author Pramunadipta, Syafiqa
Widiastuti, Ani
Wibowo, Arif
Suga, Haruhisa
Priyatmojo, Achmadi
author_facet Pramunadipta, Syafiqa
Widiastuti, Ani
Wibowo, Arif
Suga, Haruhisa
Priyatmojo, Achmadi
author_sort Pramunadipta, Syafiqa
collection PubMed
description Fusarium incarnatum-equiseti species complex (FIESC) contain over 40 members. The primer pair Smibo1FM/Semi1RM on the RPB2 partial gene has been reported to be able to identify Fusarium semitectum. The F. fujikuroi species complex (FFSC) contains more than 50 members. The F. verticillioides as a member of this complex can be identified by using VER1/VER2 primer pair on the CaM partial gene. In this research, the Smibo1FM/Semi1RM can amplify F. sulawesiense, F. hainanense, F. bubalinum, and F. tanahbumbuense, members of FIESC at 424 bp. The VER1/VER2 can amplify F. verticillioides, F. andiyazi, and F. pseudocircinatum, members of FFSC at 578 bp. Polymerase chain reaction-restriction fragment length polymorphism by using the combination of three restriction enzymes EcoRV, MspI, and HpyAV can differentiate each species of FIESC used. The two restriction enzymes HpaII and NspI can distinguish each species of FFSC used. The proper identification process is required for pathogen control in the field in order to reduce crop yield loss.
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spelling pubmed-93439092022-08-03 Development of PCR-RFLP Technique for Identify Several Members of Fusarium incarnatum-equiseti Species Complex and Fusarium fujikuroi Species Complex Pramunadipta, Syafiqa Widiastuti, Ani Wibowo, Arif Suga, Haruhisa Priyatmojo, Achmadi Plant Pathol J Note Fusarium incarnatum-equiseti species complex (FIESC) contain over 40 members. The primer pair Smibo1FM/Semi1RM on the RPB2 partial gene has been reported to be able to identify Fusarium semitectum. The F. fujikuroi species complex (FFSC) contains more than 50 members. The F. verticillioides as a member of this complex can be identified by using VER1/VER2 primer pair on the CaM partial gene. In this research, the Smibo1FM/Semi1RM can amplify F. sulawesiense, F. hainanense, F. bubalinum, and F. tanahbumbuense, members of FIESC at 424 bp. The VER1/VER2 can amplify F. verticillioides, F. andiyazi, and F. pseudocircinatum, members of FFSC at 578 bp. Polymerase chain reaction-restriction fragment length polymorphism by using the combination of three restriction enzymes EcoRV, MspI, and HpyAV can differentiate each species of FIESC used. The two restriction enzymes HpaII and NspI can distinguish each species of FFSC used. The proper identification process is required for pathogen control in the field in order to reduce crop yield loss. Korean Society of Plant Pathology 2022-06 2022-06-01 /pmc/articles/PMC9343909/ /pubmed/35678059 http://dx.doi.org/10.5423/PPJ.NT.12.2021.0184 Text en © The Korean Society of Plant Pathology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Note
Pramunadipta, Syafiqa
Widiastuti, Ani
Wibowo, Arif
Suga, Haruhisa
Priyatmojo, Achmadi
Development of PCR-RFLP Technique for Identify Several Members of Fusarium incarnatum-equiseti Species Complex and Fusarium fujikuroi Species Complex
title Development of PCR-RFLP Technique for Identify Several Members of Fusarium incarnatum-equiseti Species Complex and Fusarium fujikuroi Species Complex
title_full Development of PCR-RFLP Technique for Identify Several Members of Fusarium incarnatum-equiseti Species Complex and Fusarium fujikuroi Species Complex
title_fullStr Development of PCR-RFLP Technique for Identify Several Members of Fusarium incarnatum-equiseti Species Complex and Fusarium fujikuroi Species Complex
title_full_unstemmed Development of PCR-RFLP Technique for Identify Several Members of Fusarium incarnatum-equiseti Species Complex and Fusarium fujikuroi Species Complex
title_short Development of PCR-RFLP Technique for Identify Several Members of Fusarium incarnatum-equiseti Species Complex and Fusarium fujikuroi Species Complex
title_sort development of pcr-rflp technique for identify several members of fusarium incarnatum-equiseti species complex and fusarium fujikuroi species complex
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343909/
https://www.ncbi.nlm.nih.gov/pubmed/35678059
http://dx.doi.org/10.5423/PPJ.NT.12.2021.0184
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