A modified CUT&RUN-seq technique for qPCR analysis of chromatin-protein interactions

Chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) even with optimization may give low signal-to-background ratio and spatial resolution. Here, we adapted Cleavage Under Targets and Release Using Nuclease (CUT&RUN) (originally developed by the Henikoff group) to develop CUT&...

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Detalles Bibliográficos
Autores principales: Panday, Arvind, Elango, Rajula, Willis, Nicholas A., Scully, Ralph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344018/
https://www.ncbi.nlm.nih.gov/pubmed/35928003
http://dx.doi.org/10.1016/j.xpro.2022.101529

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344018/
https://www.ncbi.nlm.nih.gov/pubmed/35928003
http://dx.doi.org/10.1016/j.xpro.2022.101529

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