Protocol for genome-wide CRISPR knockout screens of bacterial cytotoxins in HeLa cells

CRISPR screening is a powerful tool to identify host factors for pathogenic agents including viruses and bacterial toxins. Here, we present a protocol to conduct a genome-scale CRISPR screen on HeLa cells for host factors involved in the toxin action of Clostridioides difficile TcdB4. We describe in...

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Detalles Bibliográficos
Autores principales: Yang, Qi, Zhou, Yao, He, Liuqing, Zhang, Yuanyuan, Tao, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344021/
https://www.ncbi.nlm.nih.gov/pubmed/35928004
http://dx.doi.org/10.1016/j.xpro.2022.101595

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344021/
https://www.ncbi.nlm.nih.gov/pubmed/35928004
http://dx.doi.org/10.1016/j.xpro.2022.101595

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