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Protocol for genome-wide CRISPR knockout screens of bacterial cytotoxins in HeLa cells
CRISPR screening is a powerful tool to identify host factors for pathogenic agents including viruses and bacterial toxins. Here, we present a protocol to conduct a genome-scale CRISPR screen on HeLa cells for host factors involved in the toxin action of Clostridioides difficile TcdB4. We describe in...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344021/ https://www.ncbi.nlm.nih.gov/pubmed/35928004 http://dx.doi.org/10.1016/j.xpro.2022.101595 |