Protocols for protein-DNA binding analysis of a zinc finger transcription factor bound to its cognate promoter

Here, we describe protocols to interrogate the binding of the zinc fingers of the transcription factor ZBTB7A to the fetal γ-globin (HBG) promoter. We detail the steps for performing electrophoretic mobility shift assays (EMSAs), X-ray crystallography, and isothermal titration calorimetry (ITC) to e...

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Detalles Bibliográficos
Autores principales: Ly, Lana C., Yang, Yang, Li, Fudong, Crossley, Merlin, Shi, Yunyu, Quinlan, Kate G.R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344028/
https://www.ncbi.nlm.nih.gov/pubmed/35928006
http://dx.doi.org/10.1016/j.xpro.2022.101598

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344028/
https://www.ncbi.nlm.nih.gov/pubmed/35928006
http://dx.doi.org/10.1016/j.xpro.2022.101598

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