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Intracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutions

BACKGROUND: Antibody-secreting cells are terminally differentiated B cells that play a critical role in humoral immunity through immunoglobulin secretion along with possessing the potential to be long-lived. It is now appreciated that ASCs regulate multiple aspects of biology through the secretion o...

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Detalles Bibliográficos
Autores principales: Renner, Patrick, Crone, Michael, Kornas, Matthew, Pioli, KimAnh T, Pioli, Peter D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344851/
https://www.ncbi.nlm.nih.gov/pubmed/35928457
http://dx.doi.org/10.1093/abt/tbac013