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Intracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutions
BACKGROUND: Antibody-secreting cells are terminally differentiated B cells that play a critical role in humoral immunity through immunoglobulin secretion along with possessing the potential to be long-lived. It is now appreciated that ASCs regulate multiple aspects of biology through the secretion o...
Autores principales: | Renner, Patrick, Crone, Michael, Kornas, Matthew, Pioli, KimAnh T, Pioli, Peter D |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344851/ https://www.ncbi.nlm.nih.gov/pubmed/35928457 http://dx.doi.org/10.1093/abt/tbac013 |
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